BMRB Entry 4309

Name: Backbone NMR Assignment and Secondary Structure of the 19 kDa Hemophore HasA
Published: 1999-08-12

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4309

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone NMR Assignment and Secondary Structure of the 19 kDa Hemophore HasA

Deposition date:

1999-02-18

Original release date:

1999-08-12

Authors:

Izadi-Pruneyre, Nadia; Wolff, Nicolas; Castagne, Claire; Czisch, Michael; Wandersman, Cecile; Delepierre, Muriel; Lecroisey, Anne

Citation:

Citation: Izadi-Pruneyre, Nadia; Wolff, Nicolas; Castagne, Claire; Czisch, Michael; Wandersman, Cecile; Delepierre, Muriel; Lecroisey, Anne. "Backbone NMR Assignment and Secondary Structure of the 19 kDa Hemophore HasA" J. Biomol. NMR 14, 193-194 (1999).

Assembly members:

Assembly members:
HasA, polymer, 178 residues, 18284.7 Da.

Natural source:

Natural source: Common Name: Enterobacteria Taxonomy ID: 615 Superkingdom: Eubacteria Kingdom: not available Genus/species: Serratia marcescens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSYC34

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HasA: AFSVNYDSSFGGYSIHDYLG QWASTFGDVNHTNGNVTDAN SGGFYGGSLSGSQYAISSTA NQVTAFVAGGNLTYTLFNEP AHTLYGQLDSLSFGDGLSGG DTSPYSIQVPDVSFGGLNLS SLQAQGHDGVVHQVVYGLMS GDTGALETALNGILDDYGLS VNSTFDQVAAATAVGVQH

Data sets:

assigned_chemical_shifts
- shift_set

Data type	Count
13C chemical shifts	337
15N chemical shifts	172
1H chemical shifts	369

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HasA	1

Entities:

Entity 1, HasA 178 residues - 18284.7 Da.

1

ALA

PHE

SER

VAL

ASN

TYR

ASP

SER

PHE

2

GLY

TYR

SER

ILE

HIS

ASP

TYR

LEU

GLY

3

GLN

TRP

ALA

SER

THR

PHE

GLY

ASP

VAL

ASN

4

HIS

THR

ASN

GLY

ASN

VAL

THR

ASP

ALA

ASN

5

SER

GLY

PHE

TYR

GLY

SER

LEU

SER

6

GLY

SER

GLN

TYR

ALA

ILE

SER

THR

ALA

7

ASN

GLN

VAL

THR

ALA

PHE

VAL

ALA

GLY

8

ASN

LEU

THR

TYR

THR

LEU

PHE

ASN

GLU

PRO

9

ALA

HIS

THR

LEU

TYR

GLY

GLN

LEU

ASP

SER

10

LEU

SER

PHE

GLY

ASP

GLY

LEU

SER

GLY

11

ASP

THR

SER

PRO

TYR

SER

ILE

GLN

VAL

PRO

12

ASP

VAL

SER

PHE

GLY

LEU

ASN

LEU

SER

13

SER

LEU

GLN

ALA

GLN

GLY

HIS

ASP

GLY

VAL

14

VAL

HIS

GLN

VAL

TYR

GLY

LEU

MET

SER

15

GLY

ASP

THR

GLY

ALA

LEU

GLU

THR

ALA

LEU

16

ASN

GLY

ILE

LEU

ASP

TYR

GLY

LEU

SER

17

VAL

ASN

SER

THR

PHE

ASP

GLN

VAL

ALA

18

ALA

THR

ALA

VAL

GLY

VAL

GLN

HIS

Samples:

sample_1: HasA, [U-95% 15N], 2.0 mM

sample_2: HasA, [15N 95%]-Leu, 2.0 mM

sample_3: HasA, [U-95% 13C; U-95% 15N], 2.0 mM

Ex-condition: pH: 5.6 na; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	Ex-condition
1H-15N TOCSY-HSQC	not available	not available	Ex-condition
1H-15N NOESY-HSQC	not available	not available	Ex-condition
HNCACB	not available	not available	Ex-condition
CBCA(CO)NH	not available	not available	Ex-condition
HNCO	not available	not available	Ex-condition
1H-15N HMQC-J	not available	not available	Ex-condition

Software:

XEASY v1.2 -

NMR spectrometers:

Varian Unity 500 MHz

PDB	1B2V 1DK0 1DKH 1YBJ 2CN4 2UYD 3CSL 3CSN 3DDR
EMBL	CAA57068
GB	KFD10589 KFL05309
REF	WP_016928898
SP	Q54450
AlphaFold	Q54450