BMRB Entry 4288

Title:
Sequence-specific 1H, 13C and 15N Assignment and Secondary Structure of the Apo EH2 Domain of Mouse Eps15.
Deposition date:
1998-12-21
Original release date:
1999-10-06
Authors:
Carotenuto, Alfonso; Tessari, Marco; Whitehead, Brian; Aelen, Jan; van Bergen en Henegouwen, Paul; Vuister, Geerten
Citation:

Citation: Carotenuto, Alfonso; Tessari, Marco; Whitehead, Brian; Aelen, Jan; van Bergen en Henegouwen, Paul; Vuister, Geerten. "Sequence-specific 1H, 13C and 15N Assignment and Secondary Structure of the Apo EH2 Domain of Mouse Eps15"  J. Biomol. NMR 14, 97-98 (1999).

Assembly members:

Assembly members:
EH2 domain, polymer, 105 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGex-2T

Data sets:
Data typeCount
13C chemical shifts447
15N chemical shifts98
1H chemical shifts742

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mEps15 EH21

Entities:

Entity 1, mEps15 EH2 105 residues - Formula weight is not available

1   GLYSERGLULEUPROTRPALAVALLYSSER
2   GLUASPLYSALALYSTYRASPALAILEPHE
3   ASPSERLEUSERPROVALASPGLYPHELEU
4   SERGLYASPLYSVALLYSPROVALLEULEU
5   ASNSERLYSLEUPROVALGLUILELEUGLY
6   ARGVALTRPGLULEUSERASPILEASPHIS
7   ASPGLYLYSLEUASPARGASPGLUPHEALA
8   VALALAMETPHELEUVALTYRCYSALALEU
9   GLULYSGLUPROVALPROMETSERLEUPRO
10   PROALALEUVALPROPROSERLYSARGLYS
11   THRTRPVALVALSER

Samples:

sample_one: EH2 domain, [U-15N], 1.5 mM; H2O 95%; D2O 5%

sample_two: EH2 domain, [U-15N,U-13C], 1.5 mM; H2O 95%; D2O 5%

sample_conditions_one: pH: 5.0; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • . . 600 MHz

Related Database Links:

SWISS-PROT P42567
REF NP_031969.1 NP_001009424.1
GenBank AAH48783.2 AAA02912.1 AAP12672.1 AAP12671.1
EMBL CAM23716.1
DBJ BAF74783.1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks