BMRB Entry 4288
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4288
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Citation: Carotenuto, Alfonso; Tessari, Marco; Whitehead, Brian; Aelen, Jan; van Bergen en Henegouwen, Paul; Vuister, Geerten. "Sequence-specific 1H, 13C and 15N Assignment and Secondary Structure of the Apo
EH2 Domain of Mouse Eps15" J. Biomol. NMR 14, 97-98 (1999).
Assembly members:
EH2 domain, polymer, 105 residues, Formula weight is not available
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGex-2T
Entity Sequences (FASTA):
EH2 domain: GSELPWAVKSEDKAKYDAIF
DSLSPVDGFLSGDKVKPVLL
NSKLPVEILGRVWELSDIDH
DGKLDRDEFAVAMFLVYCAL
EKEPVPMSLPPALVPPSKRK
TWVVS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 447 |
| 15N chemical shifts | 98 |
| 1H chemical shifts | 742 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | mEps15 EH2 | 1 |
Entities:
Entity 1, mEps15 EH2 105 residues - Formula weight is not available
| 1 | GLY | SER | GLU | LEU | PRO | TRP | ALA | VAL | LYS | SER | ||||
| 2 | GLU | ASP | LYS | ALA | LYS | TYR | ASP | ALA | ILE | PHE | ||||
| 3 | ASP | SER | LEU | SER | PRO | VAL | ASP | GLY | PHE | LEU | ||||
| 4 | SER | GLY | ASP | LYS | VAL | LYS | PRO | VAL | LEU | LEU | ||||
| 5 | ASN | SER | LYS | LEU | PRO | VAL | GLU | ILE | LEU | GLY | ||||
| 6 | ARG | VAL | TRP | GLU | LEU | SER | ASP | ILE | ASP | HIS | ||||
| 7 | ASP | GLY | LYS | LEU | ASP | ARG | ASP | GLU | PHE | ALA | ||||
| 8 | VAL | ALA | MET | PHE | LEU | VAL | TYR | CYS | ALA | LEU | ||||
| 9 | GLU | LYS | GLU | PRO | VAL | PRO | MET | SER | LEU | PRO | ||||
| 10 | PRO | ALA | LEU | VAL | PRO | PRO | SER | LYS | ARG | LYS | ||||
| 11 | THR | TRP | VAL | VAL | SER |
Samples:
sample_one: EH2 domain, [U-15N], 1.5 mM; H2O 95%; D2O 5%
sample_two: EH2 domain, [U-15N,U-13C], 1.5 mM; H2O 95%; D2O 5%
sample_conditions_one: pH: 5.0; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | not available | not available | sample_conditions_one |
Software:
No software information available
NMR spectrometers:
- . . 600 MHz
Related Database Links:
| SWISS-PROT | P42567 |
| REF | NP_031969.1 NP_001009424.1 |
| GenBank | AAH48783.2 AAA02912.1 AAP12672.1 AAP12671.1 |
| EMBL | CAM23716.1 |
| DBJ | BAF74783.1 |
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