BMRB Entry 4287

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4287

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H and 15N Chemical Shift Assignments of the Unstructured Yeast Target Membrane SNARE Sso1.

Deposition date:

1998-12-18

Original release date:

1999-10-05

Authors:

Fiebig, Klaus; Brunger, Axel

Citation:

Citation: Fiebig, Klaus; Rice, Luke; Pollock, Elizabeth; Brunger, Axel. "Folding Intermediates of SNARE Complex Assembly" Nat. Struct. Biol. 6, 117-123 (1999).

Assembly members:

Assembly members:
Sso1, polymer, 268 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sso1: GSHMSYNNPYQLETPFEESY ELDEGSSAIGAEGHDFVGFM NKISQINRDLDKYDHTINQV DSLHKRLLTEVNEEQASHLR HSLDNFVAQATDLQFKLKNE IKSAQRDGIHDTNKQAQAEN SRQRFLKLIQDYRIVDSNYK EENKEQAKRQYMIIQPEATE DEVEAAISDVGGQQIFSQAL LNANRRGEAKTALAEVQARH QELLKLEKSMAELTQLFNDM EELVIEQQENVDVIDKNVED AQLDVEQGVGHTDKAVKSAR KARKNKIR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts

Data type	Count
15N chemical shifts	76
1H chemical shifts	430

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Sso1	1

Entities:

Entity 1, Sso1 268 residues - Formula weight is not available

1

GLY

SER

HIS

MET

SER

TYR

ASN

PRO

TYR

2

GLN

LEU

GLU

THR

PRO

PHE

GLU

SER

TYR

3

GLU

LEU

ASP

GLU

GLY

SER

ALA

ILE

GLY

4

ALA

GLU

GLY

HIS

ASP

PHE

VAL

GLY

PHE

MET

5

ASN

LYS

ILE

SER

GLN

ILE

ASN

ARG

ASP

LEU

6

ASP

LYS

TYR

ASP

HIS

THR

ILE

ASN

GLN

VAL

7

ASP

SER

LEU

HIS

LYS

ARG

LEU

THR

GLU

8

VAL

ASN

GLU

GLN

ALA

SER

HIS

LEU

ARG

9

HIS

SER

LEU

ASP

ASN

PHE

VAL

ALA

GLN

ALA

10

THR

ASP

LEU

GLN

PHE

LYS

LEU

LYS

ASN

GLU

11

ILE

LYS

SER

ALA

GLN

ARG

ASP

GLY

ILE

HIS

12

ASP

THR

ASN

LYS

GLN

ALA

GLN

ALA

GLU

ASN

13

SER

ARG

GLN

ARG

PHE

LEU

LYS

LEU

ILE

GLN

14

ASP

TYR

ARG

ILE

VAL

ASP

SER

ASN

TYR

LYS

15

GLU

ASN

LYS

GLU

GLN

ALA

LYS

ARG

GLN

16

TYR

MET

ILE

GLN

PRO

GLU

ALA

THR

GLU

17

ASP

GLU

VAL

GLU

ALA

ILE

SER

ASP

VAL

18

GLY

GLN

ILE

PHE

SER

GLN

ALA

LEU

19

LEU

ASN

ALA

ASN

ARG

GLY

GLU

ALA

LYS

20

THR

ALA

LEU

ALA

GLU

VAL

GLN

ALA

ARG

HIS

21

GLN

GLU

LEU

LYS

LEU

GLU

LYS

SER

MET

22

ALA

GLU

LEU

THR

GLN

LEU

PHE

ASN

ASP

MET

23

GLU

LEU

VAL

ILE

GLU

GLN

GLU

ASN

24

VAL

ASP

VAL

ILE

ASP

LYS

ASN

VAL

GLU

ASP

25

ALA

GLN

LEU

ASP

VAL

GLU

GLN

GLY

VAL

GLY

26

HIS

THR

ASP

LYS

ALA

VAL

LYS

SER

ALA

ARG

27

LYS

ALA

ARG

LYS

ASN

LYS

ILE

ARG

Samples:

sample_one: Sso1, [U-99% 15N], 1.2 mM

sample_conditions: ionic strength: 220; pH: 4.5; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N-HSQC	sample_one	not available	sample_conditions
1H-15N-NOESY-HSQC	sample_one	not available	sample_conditions
1H-15N-TOCSY-HSQC	sample_one	not available	sample_conditions

Software:

FELIX v95 - transformation

XEasy v1.3.11 - Assignment

NMR spectrometers:

Varian . 600 MHz

PDB	1FIO
DBJ	GAA26746
EMBL	CAA47959 CAA54217 CAA64255 CAA97949 CAY86728
GB	AHY77951 AJP41919 AJU23363 AJU24051 AJU24725
REF	NP_015092
SP	P32867
TPG	DAA11204
AlphaFold	P32867