BMRB Entry 4272

Title:
1H, 15N, 13C Resonance Assignments of SynaptobrevinII
Deposition date:
1998-12-01
Original release date:
1999-10-26
Authors:
Hazzard, James; Sudhof, Thomas; Rizo, Josep
Citation:

Citation: Hazzard, James; Sudhof, Thomas; Rizo, Josep. "NMR analysis of the structure of synaptobrevin and of its interaction with syntaxin"  J. Biomol. NMR 14, 203-207 (1999).

Assembly members:

Assembly members:
synaptobrevinII, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-KG

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts397
13C chemical shifts351
15N chemical shifts86

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1synaptobrevinII1

Entities:

Entity 1, synaptobrevinII 96 residues - Formula weight is not available

1   METSERALATHRALAALATHRALAPROPRO
2   ALAALAPROALAGLYGLUGLYGLYPROPRO
3   ALAPROPROPROASNLEUTHRSERASNARG
4   ARGLEUGLNGLNTHRGLNALAGLNVALASP
5   GLUVALVALASPILEMETARGVALASNVAL
6   ASPLYSVALLEUGLUARGASPGLNLYSLEU
7   SERGLULEUASPASPARGALAASPALALEU
8   GLNALAGLYALASERGLNPHEGLUTHRSER
9   ALAALALYSLEULYSARGLYSTYRTRPTRP
10   LYSASNLEULYSMETMET

Samples:

sample_one: synaptobrevinII, [U-13C; U-15N], 0.45 mM; Phosphate 60 mM; EDTA 0.5 mM

sample_conditions: pH: 6.1 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HSQCnot availablenot availablenot available
NOESY-HSQCnot availablenot availablenot available
TOCSY-HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACBnot availablenot availablenot available
(H)CBCACO(CA)HAnot availablenot availablenot available
(H)C(CO)NH-TOCSYnot availablenot availablenot available

Software:

Felix v1.1 - processing all data

NmrView v3.0 - peak analysis and assignment

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

BMRB 16508 16514
PDB
DBJ BAC41125 BAE90084 BAF82244 BAG73857
EMBL CAA12385 CAA53792 CAB43509 CDQ77958
GB AAA42321 AAA60604 AAB03463 AAB62931 AAB88138
REF NP_001027992 NP_001034578 NP_001080944 NP_001088233 NP_001272142
SP P47193 P63026 P63027 P63044 P63045
TPG DAA18807
AlphaFold P47193 P63026 P63027 P63044 P63045

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks