BMRB Entry 4272
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4272
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NMR-STAR v3 text file.
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Citation: Hazzard, James; Sudhof, Thomas; Rizo, Josep. "NMR analysis of the structure of synaptobrevin and of its interaction with
syntaxin" J. Biomol. NMR 14, 203-207 (1999).
Assembly members:
synaptobrevinII, polymer, 96 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-KG
Entity Sequences (FASTA):
synaptobrevinII: MSATAATAPPAAPAGEGGPP
APPPNLTSNRRLQQTQAQVD
EVVDIMRVNVDKVLERDQKL
SELDDRADALQAGASQFETS
AAKLKRKYWWKNLKMM
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 397 |
| 13C chemical shifts | 351 |
| 15N chemical shifts | 86 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | synaptobrevinII | 1 |
Entities:
Entity 1, synaptobrevinII 96 residues - Formula weight is not available
| 1 | MET | SER | ALA | THR | ALA | ALA | THR | ALA | PRO | PRO | ||||
| 2 | ALA | ALA | PRO | ALA | GLY | GLU | GLY | GLY | PRO | PRO | ||||
| 3 | ALA | PRO | PRO | PRO | ASN | LEU | THR | SER | ASN | ARG | ||||
| 4 | ARG | LEU | GLN | GLN | THR | GLN | ALA | GLN | VAL | ASP | ||||
| 5 | GLU | VAL | VAL | ASP | ILE | MET | ARG | VAL | ASN | VAL | ||||
| 6 | ASP | LYS | VAL | LEU | GLU | ARG | ASP | GLN | LYS | LEU | ||||
| 7 | SER | GLU | LEU | ASP | ASP | ARG | ALA | ASP | ALA | LEU | ||||
| 8 | GLN | ALA | GLY | ALA | SER | GLN | PHE | GLU | THR | SER | ||||
| 9 | ALA | ALA | LYS | LEU | LYS | ARG | LYS | TYR | TRP | TRP | ||||
| 10 | LYS | ASN | LEU | LYS | MET | MET |
Samples:
sample_one: synaptobrevinII, [U-13C; U-15N], 0.45 mM; Phosphate 60 mM; EDTA 0.5 mM
sample_conditions: pH: 6.1 na; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HSQC | not available | not available | not available |
| NOESY-HSQC | not available | not available | not available |
| TOCSY-HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCACB | not available | not available | not available |
| (H)CBCACO(CA)HA | not available | not available | not available |
| (H)C(CO)NH-TOCSY | not available | not available | not available |
Software:
Felix v1.1 - processing all data
NmrView v3.0 - peak analysis and assignment
NMR spectrometers:
- Varian Unity 500 MHz
Related Database Links:
| BMRB | 16508 16514 |
| PDB | |
| DBJ | BAC41125 BAE90084 BAF82244 BAG73857 |
| EMBL | CAA12385 CAA53792 CAB43509 CDQ77958 |
| GB | AAA42321 AAA60604 AAB03463 AAB62931 AAB88138 |
| REF | NP_001027992 NP_001034578 NP_001080944 NP_001088233 NP_001272142 |
| SP | P47193 P63026 P63027 P63044 P63045 |
| TPG | DAA18807 |
| AlphaFold | P47193 P63026 P63027 P63044 P63045 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks