BMRB Entry 4269

Name: 1H, 15N and 13C Chemical Shift Assignments for the Catalytic Core of Resolvase
Published: 1999-10-14

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PDB ID: 1ght
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4269
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C Chemical Shift Assignments for the Catalytic Core of Resolvase

Deposition date:

1998-11-18

Original release date:

1999-10-14

Authors:

Pan, Borlan; Mullen, Greg

Citation:

Citation: Pan, Borlan; Mullen, Greg. "1H, 15N and 13C Chemical Shift Assignments for the Catalytic Core of Resolvase" J. Biomol. NMR 13, 307-308 (1999).

Assembly members:

Assembly members:
resolvase, polymer, 105 residues, 11608 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
resolvase: MRLFGYARVSTSQQSLDIQV RALKDAGVKANRIFTDKASG SSSDRKGLDLLRMKVEEGDV ILVKKLDRLGRDTADMIQLI KEFDAQGVSIRFIDDGISTD GEMGK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts

Data type	Count
1H chemical shifts	696
13C chemical shifts	411
15N chemical shifts	109

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	resolvase	1

Entities:

Entity 1, resolvase 105 residues - 11608 Da.

1

MET

ARG

LEU

PHE

GLY

TYR

ALA

ARG

VAL

SER

2

THR

SER

GLN

SER

LEU

ASP

ILE

GLN

VAL

3

ARG

ALA

LEU

LYS

ASP

ALA

GLY

VAL

LYS

ALA

4

ASN

ARG

ILE

PHE

THR

ASP

LYS

ALA

SER

GLY

5

SER

ASP

ARG

LYS

GLY

LEU

ASP

LEU

6

LEU

ARG

MET

LYS

VAL

GLU

GLY

ASP

VAL

7

ILE

LEU

VAL

LYS

LEU

ASP

ARG

LEU

GLY

8

ARG

ASP

THR

ALA

ASP

MET

ILE

GLN

LEU

ILE

9

LYS

GLU

PHE

ASP

ALA

GLN

GLY

VAL

SER

ILE

10

ARG

PHE

ILE

ASP

GLY

ILE

SER

THR

ASP

11

GLY

GLU

MET

GLY

LYS

Samples:

sample_one: resolvase, [U-15N], 2.0 mM; D2O 10%; H2O 90%

sample_two: resolvase, [U-13C; U-15N], 2.0 mM

sample_conditions_one: pH: 6.5; temperature: 298 K; ionic strength: 1.0

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	not available	not available	sample_conditions_one
HNCOCA	not available	not available	sample_conditions_one
HNCACB	not available	not available	sample_conditions_one
CBCACONH	not available	not available	sample_conditions_one
HNCO,	not available	not available	sample_conditions_one
CCONH	not available	not available	sample_conditions_one
HCCONH	not available	not available	sample_conditions_one
HCCH-TOCSY	not available	not available	sample_conditions_one
1H-NOESY	not available	not available	sample_conditions_one

Software:

FELIX v95 - Data Processing

XEASY v1.3.13 - Spectral visualization and resonance assignment

NMR spectrometers:

Varian . 500 MHz
Varian . 600 MHz

PDB	1GDR 1GDT 1GHT 1HX7 2RSL
DBJ	BAA03915 BAA97879
EMBL	CAA42759 CTC93933 CUH58641
GB	AAZ05410 ABX47149 ABX47153 ACB02468 ACT33552
PRF	0812253A
REF	NP_061388 WP_001235704 WP_001749204 WP_005766999 WP_010129789
SP	P03012
AlphaFold	P03012 P03012