BMRB Entry 4264

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4264
MolProbity Validation Chart

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Title:
Complex of the amino terminal domain of enzyme I and the histidine-containing phosphocarrier protein HPr from Escherichia coli nmr, restrained regularized mean structure
Deposition date:
1998-11-04
Original release date:
2000-05-02
Authors:
Clore, G.; Garrett, D.; Gronenborn, A.
Citation:

Citation: GARRETT, D.; SEOK, Y.; PETERKOFSKY, A.; GRONENBORN, A.; CLORE, G.. "Solution structure of the 40,000 Mr phosphoryl transfer complex between the N-terminal domain of enzyme I and HPr. "  Nat. Struct. Biol. 6, 166-173 (1999).

Assembly members:

Assembly members:
Enzyme I, N-terminal, polymer, 259 residues, Formula weight is not available
Histidine Phosphocarrier, polymer, 85 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Enzyme I, N-terminal: MISGILASPGIAFGKALLLK EDEIVIDRKKISADQVDQEV ERFLSGRAKASAQLETIKTK AGETFGEEKEAIFEGHIMLL EDEELEQEIIALIKDKHMTA DAAAHEVIEGQASALEELDD EYLKERAADVRDIGKRLLRN ILGLKIIDLSAIQDEVILVA ADLTPSETAQLNLKKVLGFI TDAGGRTSHTSIMARSLELP AIVGTGSVTSQVKNDDYLIL DAVNNQVYVNPTNEVIDKMR AVQEQVASEKAELAKLKDR
Histidine Phosphocarrier: MFQQEVTITAPNGLHTRPAA QFVKEAKGFTSEITVTSNGK SASAKSLFKLQTLGLTQGTV VTISAEGEDEQKAVEHLVKL MAELE

Data typeCount
1H chemical shifts2215
13C chemical shifts2194
15N chemical shifts696

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Enzyme I, N-terminal1
2Histidine Phosphocarrier2

Entities:

Entity 1, Enzyme I, N-terminal 259 residues - Formula weight is not available

1   METILESERGLYILELEUALASERPROGLY
2   ILEALAPHEGLYLYSALALEULEULEULYS
3   GLUASPGLUILEVALILEASPARGLYSLYS
4   ILESERALAASPGLNVALASPGLNGLUVAL
5   GLUARGPHELEUSERGLYARGALALYSALA
6   SERALAGLNLEUGLUTHRILELYSTHRLYS
7   ALAGLYGLUTHRPHEGLYGLUGLULYSGLU
8   ALAILEPHEGLUGLYHISILEMETLEULEU
9   GLUASPGLUGLULEUGLUGLNGLUILEILE
10   ALALEUILELYSASPLYSHISMETTHRALA
11   ASPALAALAALAHISGLUVALILEGLUGLY
12   GLNALASERALALEUGLUGLULEUASPASP
13   GLUTYRLEULYSGLUARGALAALAASPVAL
14   ARGASPILEGLYLYSARGLEULEUARGASN
15   ILELEUGLYLEULYSILEILEASPLEUSER
16   ALAILEGLNASPGLUVALILELEUVALALA
17   ALAASPLEUTHRPROSERGLUTHRALAGLN
18   LEUASNLEULYSLYSVALLEUGLYPHEILE
19   THRASPALAGLYGLYARGTHRSERHISTHR
20   SERILEMETALAARGSERLEUGLULEUPRO
21   ALAILEVALGLYTHRGLYSERVALTHRSER
22   GLNVALLYSASNASPASPTYRLEUILELEU
23   ASPALAVALASNASNGLNVALTYRVALASN
24   PROTHRASNGLUVALILEASPLYSMETARG
25   ALAVALGLNGLUGLNVALALASERGLULYS
26   ALAGLULEUALALYSLEULYSASPARG

Entity 2, Histidine Phosphocarrier 85 residues - Formula weight is not available

1   METPHEGLNGLNGLUVALTHRILETHRALA
2   PROASNGLYLEUHISTHRARGPROALAALA
3   GLNPHEVALLYSGLUALALYSGLYPHETHR
4   SERGLUILETHRVALTHRSERASNGLYLYS
5   SERALASERALALYSSERLEUPHELYSLEU
6   GLNTHRLEUGLYLEUTHRGLNGLYTHRVAL
7   VALTHRILESERALAGLUGLYGLUASPGLU
8   GLNLYSALAVALGLUHISLEUVALLYSLEU
9   METALAGLULEUGLU

Samples:

sample_one: Enzyme I, N-terminal mM; Histidine Phosphocarrier mM; D2O%; H2O%

sample_two: Enzyme I, N-terminal, [U-2H], mM; Histidine Phosphocarrier, [U-2H], mM; D2O%; H2O%

sample_cond_1: pH: 7.0; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
(1) TRIPLE RESONANCE FOR ASSIGNMENT OF PROTEIN.not availablenot availablenot available
(2) QUANTITATIVE J CORRELATION FOR COUPLING CONSTANTS.not availablenot availablenot available
(3) 3D AND 4D HETERONUCLEAR SEPARATED AND FILTERED NOE Enot availablenot availablenot available
(4) IPAP EXPTS FOR DIPOLAR COUPLINGS DIPOLAR COUPLINGS WERE MEASURED IN Anot availablenot availablenot available
NEMATIC PHASE OF A CO SUSPENSION OF PHAGE FD (CLORE ET AL. 1998 J. AM. CHEM.not availablenot availablenot available
SOC"not availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • BRUKER DMX 500 MHz
  • BRUKER DMX 600 MHz
  • BRUKER DMX 750 MHz

Related Database Links:

BMRB 17095 19958 25731 4106 29 2060 2371
PDB
DBJ BAA16290 BAB36711 BAG78231 BAI26669 BAI31701 BAB36710 BAB92987 BAA16289
EMBL CAP76888 CAQ32794 CAQ88296 CAQ99314 CAR03878 CAD07663 CAA32865 CAA35818
GB AAA24385 AAA24441 AAC75469 AAG57535 AAN43978
REF NP_311315 NP_416911 NP_708271 WP_000112669 WP_000328837 NP_456968 NP_311314 NP_416910 AP_003009 NP_288977
SP P08839
SWISS-PROT P0AA07 P0AA08 P0AA05 P0AA06 P0AA04
PIR AE0810
PRF 1107231B
GenBank AAA24440 AAA27052 AAA24384 AAA24438 AAA23655
AlphaFold P08839 P08839

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