BMRB Entry 4258

Title:
1H Chemical Shift Assignments of the Palindromic 12mer d(TTTGACGCGTCAA)2 (MCB12T) which Contains the Recognition Site for the Yeast Transcription Factor Mbp1.
Deposition date:
1998-10-29
Original release date:
1999-11-23
Authors:
McIntosh, Pauline; Taylor, Ian; Smerdon, Steven; Frenkiel, Thomas; Lane, Andrew
Citation:

Citation: McIntosh, Pauline; Taylor, Ian; Smerdon, Steven; Frenkiel, Thomas; Lane, Andrew. "1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from Saccharomyces cerevisiae in Both Its Free and DNA Bound Forms and H1 Assignments of the Free DNA"  J. Biomol. NMR 13, 397-398 (1999).

Assembly members:

Assembly members:
12MER containing the mlu1 cell cycle box, polymer, 13 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: chemically_synthesized

Entity Sequences (FASTA):

Entity Sequences (FASTA):
12MER containing the mlu1 cell cycle box: TTTGACGCGTCAA

Data sets:
Data typeCount
1H chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DNA STRAND 11
2DNA STRAND 21

Entities:

Entity 1, DNA STRAND 1 13 residues - Formula weight is not available

1   DTDTDTDGDADCDGDCDGDT
2   DCDADA

Samples:

sample_one: 12MER containing the mlu1 cell cycle box 1.0 mM; Na phosphate 40 mM; NaCl 100 mM; D2O 10%; H2O 90%

sample_conditions: pH: 7.6; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz