BMRB Entry 4255

Name: Human Translation Initiation Factor eIF1
Published: 1999-11-23

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PDB ID: 2if1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4255
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Human Translation Initiation Factor eIF1

Deposition date:

1998-10-27

Original release date:

1999-11-23

Authors:

Fletcher, C.; Pestova, T.; Hellen, C.; Wagner, G.

Citation:

Citation: Fletcher, C.; Pestova, T.; Hellen, C.; Wagner, G.. "Structure and interactions of the translation initiation factor eIF1" EMBO J. 4, 2631-2637 (1999).

Assembly members:

Assembly members:
eIF, polymer, 126 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
eIF: MRGSHHHHHHTDPMSAIQNL HSFDPFADASKGDDLLPAGT EDYIHIRIQQRNGRKTLTTV QGIADDYDKKKLVKAFKKKF ACNGTVIEHPEYGEVIQLQG DQRKNICQFLVEIGLAKDDQ LKVHGF

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts

Data type	Count
13C chemical shifts	452
15N chemical shifts	128
1H chemical shifts	730

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	eIF1	1

Entities:

Entity 1, eIF1 126 residues - Formula weight is not available

1

MET

ARG

GLY

SER

HIS

2

THR

ASP

PRO

MET

SER

ALA

ILE

GLN

ASN

LEU

3

HIS

SER

PHE

ASP

PRO

PHE

ALA

ASP

ALA

SER

4

LYS

GLY

ASP

LEU

PRO

ALA

GLY

THR

5

GLU

ASP

TYR

ILE

HIS

ILE

ARG

ILE

GLN

6

ARG

ASN

GLY

ARG

LYS

THR

LEU

THR

VAL

7

GLN

GLY

ILE

ALA

ASP

TYR

ASP

LYS

8

LYS

LEU

VAL

LYS

ALA

PHE

LYS

PHE

9

ALA

CYS

ASN

GLY

THR

VAL

ILE

GLU

HIS

PRO

10

GLU

TYR

GLY

GLU

VAL

ILE

GLN

LEU

GLN

GLY

11

ASP

GLN

ARG

LYS

ASN

ILE

CYS

GLN

PHE

LEU

12

VAL

GLU

ILE

GLY

LEU

ALA

LYS

ASP

GLN

13

LEU

LYS

VAL

HIS

GLY

PHE

Samples:

sample_one: eIF, [U-100% 15N], 1 – 2 mM; Sodium Phosphate 50 mM; dtt 1 mM; EDTA 1 mM; Azide 0.1 mM; H2O 90%; D2O 10%

sample_two: eIF, [U-100% 15N; U-100% 13C], 1 – 2 mM; Sodium Phosphate 50 mM; dtt 1 mM; EDTA 1 mM; Azide 0.1 mM; H2O 90%; D2O 10%

sample_conditions_one: ionic strength: 400 mM; pH: 6.0; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

No software information available

NMR spectrometers:

Varian UNITYplus 400 MHz
Varian INOVA 750 MHz

PDB	2IF1 4KZX 4KZY
DBJ	BAE28300 BAE29876 BAE30086 BAE30515 BAE31024
EMBL	CAA90519 CAD66615 CAG33332 CAH89503
GB	AAA60602 AAD19900 AAD52028 AAH03463 AAH05118
REF	NP_001014884 NP_001099307 NP_001124650 NP_001161207 NP_001185535
SP	P41567 P48024 P51971 Q5E938 Q5RFF4
TPG	DAA18448 DAA31349
AlphaFold	P41567 P48024 P51971 Q5E938 Q5RFF4