BMRB Entry 4254

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PDB ID: 1l3g
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4254
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from Sassharomyces cerevisiae in Both Its Free and DNA Bound Forms and 1H Assignments of the Free DNA

Deposition date:

1998-10-27

Original release date:

1999-10-26

Authors:

McIntosh, Pauline; Taylor, Ian; Smerdon, Steven; Frenkiel, Thomas; Lane, Andrew

Citation:

Citation: McIntosh, Pauline; Taylor, Ian; Smerdon, Steven; Frenkiel, Thomas; Lane, Andrew. "1H, 15N and 13C Assignments of the DNA Binding Domain of Transcription Factor Mbp1 from Saccharomyces cerevisiae in Both Its Free and DNA Bound Forms and 1H Assignments of the Fee DNA" J. Biomol. NMR 13, 397-398 (1999).

Assembly members:

Assembly members:
MLU1-BOX binding protein, polymer, 135 residues, 15436.7 Da.

Natural source:

Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Vector: pET22B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MLU1-BOX binding protein: SNQIYSARYSGVDVYEFIHS TGSIMKRKKDDWVNATHILK AANFAKAKRTRILEKEVLKE THEKVQGGFGKYQGTWVPLN IAKQLAEKFSVYDQLKPLFD FTQTDGSASPPPAPKHHHAS KVDKLAAALEHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts

Data type	Count
13C chemical shifts	242
15N chemical shifts	126
1H chemical shifts	488

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Mbp1	1

Entities:

Entity 1, Mbp1 135 residues - 15436.7 Da.

1

SER

ASN

GLN

ILE

TYR

SER

ALA

ARG

TYR

SER

2

GLY

VAL

ASP

VAL

TYR

GLU

PHE

ILE

HIS

SER

3

THR

GLY

SER

ILE

MET

LYS

ARG

LYS

ASP

4

ASP

TRP

VAL

ASN

ALA

THR

HIS

ILE

LEU

LYS

5

ALA

ASN

PHE

ALA

LYS

ALA

LYS

ARG

THR

6

ARG

ILE

LEU

GLU

LYS

GLU

VAL

LEU

LYS

GLU

7

THR

HIS

GLU

LYS

VAL

GLN

GLY

PHE

GLY

8

LYS

TYR

GLN

GLY

THR

TRP

VAL

PRO

LEU

ASN

9

ILE

ALA

LYS

GLN

LEU

ALA

GLU

LYS

PHE

SER

10

VAL

TYR

ASP

GLN

LEU

LYS

PRO

LEU

PHE

ASP

11

PHE

THR

GLN

THR

ASP

GLY

SER

ALA

SER

PRO

12

PRO

ALA

PRO

LYS

HIS

ALA

SER

13

LYS

VAL

ASP

LYS

LEU

ALA

LEU

GLU

14

HIS

Samples:

sample_one: MLU1-BOX binding protein, [U-15N], 0.8 – 1.3 mM

sample_two: MLU1-BOX binding protein, [U-15N;U-13C], 0.8 – 1.3 mM

sample_three: MLU1-BOX binding protein0.8 – 1.3 mM

sample_conditions_one: pH: 7.6; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	not available	not available	sample_conditions_one

Software:

No software information available

BMRB	4256
PDB	1BM8 1L3G
DBJ	GAA22190
EMBL	CAA52271 CAA98618 CAY78452
GB	AAS64332 AAS64333 AAS64335 AAS64336 AAS64338
REF	NP_010227
SP	P39678
TPG	DAA11800
AlphaFold	P39678