BMRB Entry 4249

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PDB ID: 1xpa
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4249
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the DNA- and RPA-binding Domain of the Human Repair Factor XPA

Deposition date:

1998-10-17

Original release date:

1999-08-06

Authors:

Ikegami, Takahisa; Kuraoka, Isao; Saijo, Masafumi; Kodo, Naohiko; Kyogoku, Yoshimasa; Morikawa, Kosuke; Tanaka, Kiyoji; Shirakawa, Masahiro

Citation:

Citation: Ikegami, Takahisa; Kuraoka, Isao; Saijo, Masafumi; Kodo, Naohiko; Kyogoku, Yoshimasa; Morikawa, Kosuke; Tanaka, Kiyoji; Shirakawa, Masahiro. "Solution Structure of the DNA- and RPA-binding Domain of the Human Repair Factor XPA" Nat. Struct. Biol. 5, 701-706 (1998).

Assembly members:

Assembly members:
XPA, polymer, 122 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
XPA: MEFDYVICEECGKEFMDSYL MNHFDLPTCDNCRDADDKHK LITKTEAKQEYLLKDCDLEK REPPLKFIVKKNPHHSQWGD MKLYLKLQIVKRSLEVWGSQ EALEEAKEVRQENREKMKQK KF

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment_one
coupling_constants
- coupling_constants

Data type	Count
13C chemical shifts	500
15N chemical shifts	120
1H chemical shifts	724
coupling constants	89

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	XPA	1
2	Zn	2

Entities:

Entity 1, XPA 122 residues - Formula weight is not available

1

MET

GLU

PHE

ASP

TYR

VAL

ILE

CYS

GLU

2

CYS

GLY

LYS

GLU

PHE

MET

ASP

SER

TYR

LEU

3

MET

ASN

HIS

PHE

ASP

LEU

PRO

THR

CYS

ASP

4

ASN

CYS

ARG

ASP

ALA

ASP

LYS

HIS

LYS

5

LEU

ILE

THR

LYS

THR

GLU

ALA

LYS

GLN

GLU

6

TYR

LEU

LYS

ASP

CYS

ASP

LEU

GLU

LYS

7

ARG

GLU

PRO

LEU

LYS

PHE

ILE

VAL

LYS

8

LYS

ASN

PRO

HIS

SER

GLN

TRP

GLY

ASP

9

MET

LYS

LEU

TYR

LEU

LYS

LEU

GLN

ILE

VAL

10

LYS

ARG

SER

LEU

GLU

VAL

TRP

GLY

SER

GLN

11

GLU

ALA

LEU

GLU

ALA

LYS

GLU

VAL

ARG

12

GLN

GLU

ASN

ARG

GLU

LYS

MET

LYS

GLN

LYS

13

LYS

PHE

Entity 2, Zn - Zn - 65.409 Da.

1

ZN

Samples:

sample_one: Human NER factor XPA, [U-13C; U-15N], 1.2 mM; zinc 0.02 mM; KCl 150 mM; DTT 10 mM; Deuterated Tris-HCl 50 mM

sample_conditions_one: pH: 7.3; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	sample_one	not available	sample_conditions_one

Software:

NMRPIPE v1.6 - Data processing

PIPP v4.0.4 - Peak picker

NMR spectrometers:

Bruker DMX500 500 MHz
Bruker DRX500 500 MHz
Bruker DRX800 800 MHz

PDB	1D4U 1XPA
DBJ	BAA03403 BAJ20553
GB	AAA92883 AAH14965 AAM18969 AAV38325 AAX29766
PRF	1701435A
REF	NP_000371 XP_001114093 XP_001156167 XP_002743160 XP_002820062
SP	P23025
AlphaFold	P23025