BMRB Entry 4247

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4247

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H Chemical Shift Assignments of the 8mer Chimeric Hybrid Duplex r(GCAGUGGC).r(RCCA)d(CTGC)

Deposition date:

1998-10-15

Original release date:

2000-03-03

Authors:

Szyperski, Thomas; Goette, Matthias; Billeter, Martin; Perola, Emanuele; Celai, Luciano; Heumann, Hermann; Wuthrich, Kurt

Citation:

Citation: Szyperski, Thomas; Goette, Matthias; Billeter, Martin; Perola, Emanuele; Celai, Luciano; Heumann, Hermann; Wuthrich, Kurt. "NMR Structure of the Chimeric Hybrid Duplex r(gcaguggc). r(gcca)d(CTGC) Comprising the tRNA-DNA Junction Formed During Initiation of HIV-1 Reverse Trancription" J. Biomol. NMR 13, 343-355 (1999).

Assembly members:

Assembly members:
CHD_strand_A, polymer, 8 residues, Formula weight is not available
CHD_strand_B_RNA, polymer, 4 residues, Formula weight is not available
CHD_strand_B_DNA, polymer, 4 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eucaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: Chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CHD_strand_A: GCAGUGGC
CHD_strand_B_RNA: GCCA
CHD_strand_B_DNA: CTGC

Data sets:

assigned_chemical_shifts

Data type	Count
1H chemical shifts	145

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CHDA	1
2	CHDB RNA	2
3	CHDB DNA	3

Entities:

Entity 1, CHDA 8 residues - Formula weight is not available

1

G

C

A

G

U

G

C

Entity 2, CHDB RNA 4 residues - Formula weight is not available

1

G

C

A

Entity 3, CHDB DNA 4 residues - Formula weight is not available

1

DC

DT

DG

DC

Samples:

sample_one: 8mer chimeric hybrid RNA/RNA-DNA duplex with tRNA-DNA junction 1.5 mM; KPO4 20 mM; NaCL 100 mM; D2O 10%; H2O 90%

sample_conditions_one: pH: 7.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	sample_one	not available	sample_conditions_one

Software:

No software information available

NMR spectrometers:

Bruker AMX 500 MHz
BRUKER AMX 600 MHz
VARIAN UNITY_PLUS 750 MHz