BMRB Entry 4246
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4246
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Citation: Zhukov, Igor; Bolewska, Krystina; Bierzynski, Andrzej. "Conservative Mutation Met8-->Leu Affects the Folding Process and Structural
Stability of Squash Trypsin Inhibitor CMTI-I" Protein Sci. 9, 273-279 (2000).
Assembly members:
CMTI-I(M8L), polymer, 29 residues, Formula weight is not available
Natural source: Common Name: squash Taxonomy ID: 3661 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Cucurbita maxima
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pLys_S
Entity Sequences (FASTA):
CMTI-I(M8L): RVCPRILLECKKDSDCLAEC
VCLEHGYCG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 198 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CMTI-I(M8L) | 1 |
Entities:
Entity 1, CMTI-I(M8L) 29 residues - Formula weight is not available
| 1 | ARG | VAL | CYS | PRO | ARG | ILE | LEU | LEU | GLU | CYS | ||||
| 2 | LYS | LYS | ASP | SER | ASP | CYS | LEU | ALA | GLU | CYS | ||||
| 3 | VAL | CYS | LEU | GLU | HIS | GLY | TYR | CYS | GLY |
Samples:
sample_one: CMTI-I(M8L) mM; NaCl 50 mM
sample_conditions_one: pH: 5.6; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_one | not available | sample_conditions_one |
| TOCSY | sample_one | not available | sample_conditions_one |
| DQF-COSY | sample_one | not available | sample_conditions_one |
Software:
XEASY v3.21 - assignment and integration of two-dimensional NMR spectra
NMR spectrometers:
- Varian UNITYplus 500 MHz
Related Database Links:
| PDB |