BMRB Entry 4242

Name: Motile Major Sperm Protein (MSP) of Ascaris suum
Published: 1999-01-25

Click here to enlarge.

PDB ID: 3msp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4242
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Motile Major Sperm Protein (MSP) of Ascaris suum

Deposition date:

1998-10-09

Original release date:

1999-01-25

Authors:

Haaf, Andreas; LeClaire, Lawrence; Roberts, Gregory; Kent, Helen; Roberts, Thomas; Stewart, Murray; Neuhaus, David

Citation:

Citation: Haaf, Andreas; LeClaire, Lawrence; Roberts, Gregory; Kent, Helen; Roberts, Thomas; Stewart, Murray; Neuhaus, David. "Solution Structure of the Motile Major Sperm Protein (MSP) of Ascaris suum - Evidence for Two Manganese Binding Sites and the Possible Role of Divalent Cations in Filament Formation." J. Mol. Biol. 284, 1611-1623 (1998).

Assembly members:

Assembly members:
Major Sperm Protein, polymer, 126 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Pig Roundworm Taxonomy ID: 6253 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Ascaris suum

Experimental source:

Experimental source: Production method: recombinant_technology Host organism: Escherichia coli Vector: pET11d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Major Sperm Protein: AQSVPPGDINTQPSQKIVFN APYDDKHTYHIKITNAGGRR IGWAIKTTNMRRLSVDPPCG VLDPKEKVLMAVSCDTFNAA TEDLNNDRITIEWTNTPDGA AKQFRREWFQGDGMVRRKNL PIEYNL

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment_one

Data type	Count
13C chemical shifts	410
15N chemical shifts	132
1H chemical shifts	825

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MSP subunit A	1
2	MSP subunit B	1

Entities:

Entity 1, MSP subunit A 126 residues - Formula weight is not available

1

ALA

GLN

SER

VAL

PRO

GLY

ASP

ILE

ASN

2

THR

GLN

PRO

SER

GLN

LYS

ILE

VAL

PHE

ASN

3

ALA

PRO

TYR

ASP

LYS

HIS

THR

TYR

HIS

4

ILE

LYS

ILE

THR

ASN

ALA

GLY

ARG

5

ILE

GLY

TRP

ALA

ILE

LYS

THR

ASN

MET

6

ARG

LEU

SER

VAL

ASP

PRO

CYS

GLY

7

VAL

LEU

ASP

PRO

LYS

GLU

LYS

VAL

LEU

MET

8

ALA

VAL

SER

CYS

ASP

THR

PHE

ASN

ALA

9

THR

GLU

ASP

LEU

ASN

ASP

ARG

ILE

THR

10

ILE

GLU

TRP

THR

ASN

THR

PRO

ASP

GLY

ALA

11

ALA

LYS

GLN

PHE

ARG

GLU

TRP

PHE

GLN

12

GLY

ASP

GLY

MET

VAL

ARG

LYS

ASN

LEU

13

PRO

ILE

GLU

TYR

ASN

LEU

Samples:

sample_one: Major Sperm Protein2.5 – 3.5 mM; D2O 5%; H2O 95%

sample_two: Major Sperm Protein, [U-98%15N], 2.5 – 3.5 mM; D2O 5%; H2O 95%

sample_three: Major Sperm Protein, [U-98%13C;U-98%15N], 2.5 – 3.5 mM; D2O 5%; H2O 95%

sample_conditions_one: pH: 4.5; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
NOESY	not available	not available	not available
TOCSY	not available	not available	not available
DQ-correlation	not available	not available	not available
DQF-COSY	not available	not available	not available
3D 15N HSQC-NOESY	not available	not available	not available
3D 13C HSQC-NOESY	not available	not available	not available
HCCH	not available	not available	not available

Software:

FELIX v2.30 - processing and analysis

NMR spectrometers:

Bruker AMX500 500 MHz
Bruker DMX600 600 MHz

PDB	1MSP 2BVU 3MSP
EMBL	CAA63933
GB	AAA29375 AAB23264 AAP76176 ERG81453
SP	P27439
AlphaFold	P27439