BMRB Entry 4237

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4237

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Title:
Backbone and side-chain 1H, 15N, and 13C Assignments for the Topological Specificity Domain of the MinE cell Division Protein
Deposition date:
1998-10-06
Original release date:
1999-12-23
Authors:
King, Glenn; Pan, Borlan; Maciejewski, Mark; Rowland, Susan; Rothfield, Lawrence; Mullen, Gregory
Citation:

Citation: King, Glenn; Pan, Borlan; Maciejewski, Mark; Rowland, Susan; Rothfield, Lawrence; Mullen, Gregory. "Backbone and side-chain 1H, 15N, and 13C Assignments for the Topological Specificity Domain of the MinE cell Division Protein"  J. Biomol. NMR 13, 395-396 (1999).

Assembly members:

Assembly members:
MinE, polymer, 58 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MinE: RSDAEPHYLPQLRKDILEVI CKYVQIDPEMVTVQLEQKDG DISILELNVTLPEAEELK

Data sets:
Data typeCount
13C chemical shifts254
15N chemical shifts57
1H chemical shifts445

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MinE subunit 11
2MinE subunit 21

Entities:

Entity 1, MinE subunit 1 58 residues - Formula weight is not available

1   ARGSERASPALAGLUPROHISTYRLEUPRO
2   GLNLEUARGLYSASPILELEUGLUVALILE
3   CYSLYSTYRVALGLNILEASPPROGLUMET
4   VALTHRVALGLNLEUGLUGLNLYSASPGLY
5   ASPILESERILELEUGLULEUASNVALTHR
6   LEUPROGLUALAGLUGLULEULYS

Samples:

Sample1: MinE, [U-15N], 2.9 – 3.5 mM

Sample2: MinE, [U-15N; U-13C], 2.9 – 3.5 mM

Ex-cond_1: ionic strength: 0.075 M; pH: 5.7; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOnot availablenot availableEx-cond_1
3D HNCACBnot availablenot availableEx-cond_1
3D CBCA(CO)NHnot availablenot availableEx-cond_1
3D HCCH-TOCSYnot availablenot availableEx-cond_1
3D TOCSY-HSQCnot availablenot availableEx-cond_1
3D HC(CO)NH-TOCSYnot availablenot availableEx-cond_1
3D C(CO)NH-TOCSYnot availablenot availableEx-cond_1
3D 13C NOESY-HSQCnot availablenot availableEx-cond_1
3D 15N NOESY-HSQCnot availablenot availableEx-cond_1

Software:

FELIX v95 - data processing

XEASY v1.3.13 - spectral analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA36008 BAB35091 BAG76744 BAI24985 BAI30111
EMBL CAD05499 CAP75708 CAQ31676 CAQ89293 CAQ98052
GB AAB59063 AAC74258 AAG56025 AAL20731 AAN42777
PIR AI0724
REF NP_309695 NP_415692 NP_456323 NP_460772 NP_707070
SP A1AA98 A4WBG2 A7ZKU5 A7ZZA8 A8AFQ7
AlphaFold A1AA98 A4WBG2 A7ZKU5 A7ZZA8 A8AFQ7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks