BMRB Entry 4217
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4217
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Ohno, A.; Tate, S.; Seeram, S.; Hiraga, K.; Oda, K.; Swindells, M.; Kainosho, M.. "NMR structure of the streptomyces metalloproteinase inhibitor, SMPI, isolated
from Streptomyces nigrescens TK-23: another example of an ancestral beta
gamma-crystallin precursor structure." J. Mol. Biol. 282, 421-433 (1998).
Assembly members:
metalloproteinase inhibitor, polymer, 102 residues, Formula weight is not available
Natural source: Common Name: Streptomyces nigrescens Taxonomy ID: 1920 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptomyces nigrescens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
metalloproteinase inhibitor: APSCPAGSLCTYSGTGLSGA
RTVIPASDMEKAGTDGVKLP
ASARSFANGTHFTLRYGPAR
KVTCVRFPCYQYATVGKVAP
GAQLRSLPSPGATVTVGQDL
GD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 191 |
| 15N chemical shifts | 97 |
| 1H chemical shifts | 623 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SMPI | 1 |
Entities:
Entity 1, SMPI 102 residues - Formula weight is not available
| 1 | ALA | PRO | SER | CYS | PRO | ALA | GLY | SER | LEU | CYS | ||||
| 2 | THR | TYR | SER | GLY | THR | GLY | LEU | SER | GLY | ALA | ||||
| 3 | ARG | THR | VAL | ILE | PRO | ALA | SER | ASP | MET | GLU | ||||
| 4 | LYS | ALA | GLY | THR | ASP | GLY | VAL | LYS | LEU | PRO | ||||
| 5 | ALA | SER | ALA | ARG | SER | PHE | ALA | ASN | GLY | THR | ||||
| 6 | HIS | PHE | THR | LEU | ARG | TYR | GLY | PRO | ALA | ARG | ||||
| 7 | LYS | VAL | THR | CYS | VAL | ARG | PHE | PRO | CYS | TYR | ||||
| 8 | GLN | TYR | ALA | THR | VAL | GLY | LYS | VAL | ALA | PRO | ||||
| 9 | GLY | ALA | GLN | LEU | ARG | SER | LEU | PRO | SER | PRO | ||||
| 10 | GLY | ALA | THR | VAL | THR | VAL | GLY | GLN | ASP | LEU | ||||
| 11 | GLY | ASP |
Samples:
sample_1: metalloproteinase inhibitor mM
sample_cond_1: pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| Heteronuclear multi-dimensional experiment | sample_1 | not available | sample_cond_1 |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
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