BMRB Entry 4208
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4208
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Blackledge, M.; Medvedeva, S.; Poncin, M.; Guerlesquin, F.; Bruschi, M.; Marion, D.. "Structure and Dynamics of Ferrocytochrome c553 from Desulfovibrio vulgaris
Studied by Spectroscopy and Restrained Molecular Dynamics NMR" J. Mol. Biol. 245, 661-681 (1995).
PubMed: 7844834
Assembly members:
CytC(Y64A), polymer, 79 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.
Natural source: Common Name: Desulfovibrio desulfuricans Taxonomy ID: 876 Superkingdom: Bacteria Kingdom: not available Genus/species: Desulfovibrio desulfuricans
Experimental source: Production method: recombinant technology Host organism: Desulfovibrio desulfuricans
Entity Sequences (FASTA):
CytC(Y64A): ADGAALYKSCIGCHGADGSK
AAMGSAKPVKGQGAEELYKK
MKGYADGSYGGERKAMMTNA
VKKASDEELKALADYMSKL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 473 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CytC(Y64A) | 1 |
| 2 | heme | 2 |
Entities:
Entity 1, CytC(Y64A) 79 residues - Formula weight is not available
| 1 | ALA | ASP | GLY | ALA | ALA | LEU | TYR | LYS | SER | CYS | ||||
| 2 | ILE | GLY | CYS | HIS | GLY | ALA | ASP | GLY | SER | LYS | ||||
| 3 | ALA | ALA | MET | GLY | SER | ALA | LYS | PRO | VAL | LYS | ||||
| 4 | GLY | GLN | GLY | ALA | GLU | GLU | LEU | TYR | LYS | LYS | ||||
| 5 | MET | LYS | GLY | TYR | ALA | ASP | GLY | SER | TYR | GLY | ||||
| 6 | GLY | GLU | ARG | LYS | ALA | MET | MET | THR | ASN | ALA | ||||
| 7 | VAL | LYS | LYS | ALA | SER | ASP | GLU | GLU | LEU | LYS | ||||
| 8 | ALA | LEU | ALA | ASP | TYR | MET | SER | LYS | LEU |
Entity 2, heme - C34 H32 Fe N4 O4 - 616.487 Da.
| 1 | HEM |
Samples:
sample_one: CytC(Y64A) mM
sample_conditions_one: ionic strength: 10 mM; pH: 5.9; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_one | not available | sample_conditions_one |
| HOHAHA | sample_one | not available | sample_conditions_one |
Software:
No software information available
NMR spectrometers:
- Bruker AMX 600 MHz