BMRB Entry 4199

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PDB ID: 3nla
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4199
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Determination of the Solution Structure of the N-Domain Plus Linker of Antarctic Eel Pout Antifreeze Protein RD3

Deposition date:

1998-09-10

Original release date:

1999-12-22

Authors:

Miura, Kazunori; Ohgiya, Satoru; Hoshino, Tamotsu; Nemoto, Nobuaki; Odaira, Masato; Nitta, Katsutoshi; Tsuda, Sakae

Citation:

Citation: Miura, Kazunori; Ohgiya, Satoru; Hoshino, Tamotsu; Nemoto, Nobuaki; Odaira, Masato; Nitta, Katsutoshi; Tsuda, Sakae. "Determination of the Solution Structure of the N-Domain Plus Linker of Antarctic Eel Pout Antifreeze Protein RD3" J. Biochem. 126, 387-394 (1999).

Assembly members:

Assembly members:
N-term. domain with linker Type III Antifreeze protein RD3, polymer, 73 residues, Formula weight is not available

Natural source:

Natural source: Common Name: antarctic eel pout Taxonomy ID: 8201 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Lycodichthys dearborni

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pKK223-3UC

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N-term. domain with linker Type III Antifreeze protein RD3: NKASVVANQLIPINTALTLI MMKAEVVTPMGIPAEEIPNL VGMQVNRAVPLGTTLMPDMV KNYEDGTTSPGLK

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment_one
coupling_constants
- coupling_constants_1

Data type	Count
15N chemical shifts	74
1H chemical shifts	525
coupling constants	50

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RD3-N1	1

Entities:

Entity 1, RD3-N1 73 residues - Formula weight is not available

1

ASN

LYS

ALA

SER

VAL

ALA

ASN

GLN

LEU

2

ILE

PRO

ILE

ASN

THR

ALA

LEU

THR

LEU

ILE

3

MET

LYS

ALA

GLU

VAL

THR

PRO

MET

4

GLY

ILE

PRO

ALA

GLU

ILE

PRO

ASN

LEU

5

VAL

GLY

MET

GLN

VAL

ASN

ARG

ALA

VAL

PRO

6

LEU

GLY

THR

LEU

MET

PRO

ASP

MET

VAL

7

LYS

ASN

TYR

GLU

ASP

GLY

THR

SER

PRO

8

GLY

LEU

LYS

Samples:

sample_one: N-term. domain with linker Type III Antifreeze protein RD3 1.0 mM

sample_two: N-term. domain with linker Type III Antifreeze protein RD3, [U-15N], 1.0 mM

sample_conditions: ionic strength: 0.05 M; pH: 6.7; temperature: 277 K

Experiments:

Name	Sample	Sample state	Sample conditions
DQFCOSY	sample_two	not available	sample_conditions
TOCSY	sample_two	not available	sample_conditions
NOESY	sample_two	not available	sample_conditions
15N-HSQC	sample_two	not available	sample_conditions
15N_TOCSYHSQC	sample_two	not available	sample_conditions
15N-NOESYHSQC	sample_two	not available	sample_conditions

Software:

No software information available

NMR spectrometers:

Varian Unity 500 MHz
JEOL JEOL 500 MHz

PDB	1UCS 3NLA 3RDN
GB	AFU88733 AFU88737 AFU88748
PRF	2109220A
SP	P35751
AlphaFold	P35751