BMRB Entry 4185

Title:
1H Chemical Shift Assignments for Gurmarin
Deposition date:
1998-08-13
Original release date:
2008-07-16
Authors:
Fletcher, Jamie; Dingley, Andrew; King, Glenn
Citation:

Citation: Fletcher, Jamie; Dingley, Andrew; Smith, Ross; Connor, Mark; MacDonald, Christie; King, Glenn. "High-Resolution Solution Structure of Gurmarin,a Sweet-Taste-Suppressing Plant Polypeptide"  Eur. J. Biochem. 264, 525-533 (1999).

Assembly members:

Assembly members:
gurmarin, polymer, 35 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Gymnema sylvestre   Taxonomy ID: 4068   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Gymnema sylvestre

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
gurmarin: XQCVKKDELCIPYYLDCCEP LECKKVNWWDHKCIG

Data sets:
Data typeCount
1H chemical shifts268

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gmn1

Entities:

Entity 1, gmn 35 residues - Formula weight is not available

1   PCAGLNCYSVALLYSLYSASPGLULEUCYS
2   ILEPROTYRTYRLEUASPCYSCYSGLUPRO
3   LEUGLUCYSLYSLYSVALASNTRPTRPASP
4   HISLYSCYSILEGLY

Samples:

sample_one: gurmarin 2.5 mM

sample_conditions_one: pH: 2.9; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H TOCSYsample_onenot availablesample_conditions_one
1H NOESYsample_onenot availablesample_conditions_one
DQF-COSYsample_onenot availablesample_conditions_one
ECOSYsample_onenot availablesample_conditions_one

Software:

XWINMR - Spectral processing

XEASY - Spectral assignment

NMR spectrometers:

  • BRUKER AMX 600 MHz

Related Database Links:

PDB
GB AAB22380 AAB35858
SP P25810
AlphaFold P25810