BMRB Entry 4185
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4185
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Citation: Fletcher, Jamie; Dingley, Andrew; Smith, Ross; Connor, Mark; MacDonald, Christie; King, Glenn. "High-Resolution Solution Structure of Gurmarin,a Sweet-Taste-Suppressing Plant
Polypeptide" Eur. J. Biochem. 264, 525-533 (1999).
Assembly members:
gurmarin, polymer, 35 residues, Formula weight is not available
Natural source: Common Name: Gymnema sylvestre Taxonomy ID: 4068 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Gymnema sylvestre
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
gurmarin: XQCVKKDELCIPYYLDCCEP
LECKKVNWWDHKCIG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 268 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | gmn | 1 |
Entities:
Entity 1, gmn 35 residues - Formula weight is not available
| 1 | PCA | GLN | CYS | VAL | LYS | LYS | ASP | GLU | LEU | CYS | ||||
| 2 | ILE | PRO | TYR | TYR | LEU | ASP | CYS | CYS | GLU | PRO | ||||
| 3 | LEU | GLU | CYS | LYS | LYS | VAL | ASN | TRP | TRP | ASP | ||||
| 4 | HIS | LYS | CYS | ILE | GLY |
Samples:
sample_one: gurmarin 2.5 mM
sample_conditions_one: pH: 2.9; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H TOCSY | sample_one | not available | sample_conditions_one |
| 1H NOESY | sample_one | not available | sample_conditions_one |
| DQF-COSY | sample_one | not available | sample_conditions_one |
| ECOSY | sample_one | not available | sample_conditions_one |
Software:
XWINMR - Spectral processing
XEASY - Spectral assignment
NMR spectrometers:
- BRUKER AMX 600 MHz