BMRB Entry 4181
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4181
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
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Citation: Im, Sang-Choul; Liu, Gaohua; Luchinat, Claudio; Sykes, Geoffrey; Bertini, Ivano. "The Solution Structure of Parsley [2Fe-2S] Ferredoxin" Eur. J. Biochem. 258, 465-477 (1998).
Assembly members:
ferredoxin, polymer, 96 residues, Formula weight is not available
FES, non-polymer, 175.820 Da.
Natural source: Common Name: parsley Taxonomy ID: 4043 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Petroselinum scrispum
Experimental source: Production method: purified from the natural source Host organism: Petroselinum scrispum
Entity Sequences (FASTA):
ferredoxin: ATYNVKLITPDGEVEFKCDD
DVYVLDQAEEEGIDIPYSCR
ADSCSSCAGKVVSGSIDQSD
QSFKDDEQMDAGYVLTCHAY
PTSDVVIETHKEEEIV
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 570 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Fd I | 1 |
| 2 | 2Fe2S | 2 |
Entities:
Entity 1, Fd I 96 residues - Formula weight is not available
| 1 | ALA | THR | TYR | ASN | VAL | LYS | LEU | ILE | THR | PRO | ||||
| 2 | ASP | GLY | GLU | VAL | GLU | PHE | LYS | CYS | ASP | ASP | ||||
| 3 | ASP | VAL | TYR | VAL | LEU | ASP | GLN | ALA | GLU | GLU | ||||
| 4 | GLU | GLY | ILE | ASP | ILE | PRO | TYR | SER | CYS | ARG | ||||
| 5 | ALA | ASP | SER | CYS | SER | SER | CYS | ALA | GLY | LYS | ||||
| 6 | VAL | VAL | SER | GLY | SER | ILE | ASP | GLN | SER | ASP | ||||
| 7 | GLN | SER | PHE | LYS | ASP | ASP | GLU | GLN | MET | ASP | ||||
| 8 | ALA | GLY | TYR | VAL | LEU | THR | CYS | HIS | ALA | TYR | ||||
| 9 | PRO | THR | SER | ASP | VAL | VAL | ILE | GLU | THR | HIS | ||||
| 10 | LYS | GLU | GLU | GLU | ILE | VAL |
Entity 2, 2Fe2S - Fe2 S2 - 175.820 Da.
| 1 | FES |
Samples:
sample_one: ferredoxin3.5 – 4 mM
sample_conditions_one: ionic strength: 0.05 M; pH: 7; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_one | not available | sample_conditions_one |
| TOCSY | sample_one | not available | sample_conditions_one |
| DQF-COSY | sample_one | not available | sample_conditions_one |
| Inversion-recovery NOESY | sample_one | not available | sample_conditions_one |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 800 MHz
- Bruker Avance 600 MHz