BMRB Entry 4177
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4177
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Kim, K.-S.; Rajarathnam, K.; Clark-Lewis, I.; Sykes, B.. "Structural Characterization of a Monomeric Chemokine: Monocyte
Chemoattractant Protein-3" FEBS Lett. 395, 277-. (1996).
Assembly members:
Monocyte_chemoattractant_protein-3, polymer, 76 residues, Formula weight is not available
N-terminal cloning artifact, polymer, 4 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b
Entity Sequences (FASTA):
Monocyte_chemoattractant_protein-3: QPVGINTSTTCCYRFINKKI
PKQRLESYRRTTSSHCPREA
VIFKTKLDKEICADPTQKWV
QDFMKHLDKKTQTPKL
N-terminal cloning artifact: GSHM
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 247 |
| 15N chemical shifts | 81 |
| 1H chemical shifts | 566 |
| coupling constants | 68 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MCP-3 | 1 |
| 2 | N-terminal artifact | 2 |
Entities:
Entity 1, MCP-3 76 residues - Formula weight is not available
| 1 | GLN | PRO | VAL | GLY | ILE | ASN | THR | SER | THR | THR | ||||
| 2 | CYS | CYS | TYR | ARG | PHE | ILE | ASN | LYS | LYS | ILE | ||||
| 3 | PRO | LYS | GLN | ARG | LEU | GLU | SER | TYR | ARG | ARG | ||||
| 4 | THR | THR | SER | SER | HIS | CYS | PRO | ARG | GLU | ALA | ||||
| 5 | VAL | ILE | PHE | LYS | THR | LYS | LEU | ASP | LYS | GLU | ||||
| 6 | ILE | CYS | ALA | ASP | PRO | THR | GLN | LYS | TRP | VAL | ||||
| 7 | GLN | ASP | PHE | MET | LYS | HIS | LEU | ASP | LYS | LYS | ||||
| 8 | THR | GLN | THR | PRO | LYS | LEU |
Entity 2, N-terminal artifact 4 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET |
Samples:
sample_one: Monocyte_chemoattractant_protein-3, [U-95% 13C; U-95% 15N], 1.9 – 2.1 mM
sample_conditions_one: pH: 5.1; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N | sample_one | not available | sample_conditions_one |
| 1H-13C NOESY | sample_one | not available | sample_conditions_one |
| 1H-15N TOCSY | sample_one | not available | sample_conditions_one |
| HNHA | sample_one | not available | sample_conditions_one |
| CBCA(CO)NH | sample_one | not available | sample_conditions_one |
| 1H-15N NOE | sample_one | not available | sample_conditions_one |
Software:
NMRPipe v97.231.15.18 - processing
NMR spectrometers:
- . . 600 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks