BMRB Entry 4168
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4168
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NMR-STAR v3 text file.
XML gzip file.
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Citation: Huang, Wen; Dolmer, Klavs; Liao, Xiubei; Gettins, Peter. "Localization of Basic Residues Required for Receptor Binding to the Single
Alpha-helix of the Receptor Binding Domain of Human Alpha-2-macroglobulin" Protein Sci. 7, 2602-2612 (1998).
Assembly members:
Alpha-2-macroglobulin receptor binding domain, polymer, 138 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Alpha-2-macroglobulin receptor binding domain: EEFPFALGVQTLPQTCDEPK
AHTSFQISLSVSYTGSRSAS
NMAIVDVKMVSGFIPLKPTV
KMLERSNHVSRTEVSSNHVL
IYLDKVSNQTLSLFFTVLQD
VPVRDLKPAIVKVYDYYETD
EFAIAEYNAPCSKDLGNA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 138 |
| 15N chemical shifts | 130 |
| 1H chemical shifts | 271 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RBD | 1 |
Entities:
Entity 1, RBD 138 residues - Formula weight is not available
| 1 | GLU | GLU | PHE | PRO | PHE | ALA | LEU | GLY | VAL | GLN | ||||
| 2 | THR | LEU | PRO | GLN | THR | CYS | ASP | GLU | PRO | LYS | ||||
| 3 | ALA | HIS | THR | SER | PHE | GLN | ILE | SER | LEU | SER | ||||
| 4 | VAL | SER | TYR | THR | GLY | SER | ARG | SER | ALA | SER | ||||
| 5 | ASN | MET | ALA | ILE | VAL | ASP | VAL | LYS | MET | VAL | ||||
| 6 | SER | GLY | PHE | ILE | PRO | LEU | LYS | PRO | THR | VAL | ||||
| 7 | LYS | MET | LEU | GLU | ARG | SER | ASN | HIS | VAL | SER | ||||
| 8 | ARG | THR | GLU | VAL | SER | SER | ASN | HIS | VAL | LEU | ||||
| 9 | ILE | TYR | LEU | ASP | LYS | VAL | SER | ASN | GLN | THR | ||||
| 10 | LEU | SER | LEU | PHE | PHE | THR | VAL | LEU | GLN | ASP | ||||
| 11 | VAL | PRO | VAL | ARG | ASP | LEU | LYS | PRO | ALA | ILE | ||||
| 12 | VAL | LYS | VAL | TYR | ASP | TYR | TYR | GLU | THR | ASP | ||||
| 13 | GLU | PHE | ALA | ILE | ALA | GLU | TYR | ASN | ALA | PRO | ||||
| 14 | CYS | SER | LYS | ASP | LEU | GLY | ASN | ALA |
Samples:
sample_one: Alpha-2-macroglobulin receptor binding domain, [U-95% 13C; U-90%15N], 0.9 – 1.2 mM
sample_conditions_one: ionic strength: 0.4 M; pH: 5.1; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N-HSQC | sample_one | not available | not available |
| 1H-15N TOCSY | sample_one | not available | not available |
| NOESY-HSQC | sample_one | not available | not available |
| HNCA | sample_one | not available | not available |
| HNCACB | sample_one | not available | not available |
| CBCA(CO)NH | sample_one | not available | not available |
| HBHA(CO)NH | sample_one | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DMX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAD92851 BAG10847 |
| EMBL | CAH18188 CAH93278 |
| GB | AAA51551 AAA51552 AAH40071 AAQ13498 AAT02228 |
| PRF | 1009174A |
| REF | NP_000005 NP_001126929 XP_001139819 XP_003265499 XP_003804581 |
| SP | P01023 Q5R4N8 |
| AlphaFold | P01023 Q5R4N8 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks