BMRB Entry 4164
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4164
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Citation: Atkinson, R; Salah El Din, Abdel Latif; Kieffer, Bruno; Lefevre, Jean-Francois; Abdallah, Mohamed. "Bacterial Iron Transport: 1H NMR Determination of the Three-dimensional
Structure of the Gallium Complex of Pyoverdin G4R, the Peptidic Siderophore of
Pseudomonas putida G4R" Biochemistry 37, 15965-15973 (1998).
PubMed: 9843403
Assembly members:
pyoverdin G4R, polymer, 9 residues, Formula weight is not available
GALLIUM (III) ION, non-polymer, 69.723 Da.
Natural source: Common Name: Pseudomonas putida Taxonomy ID: 303 Superkingdom: Eubacteria Kingdom: not available Genus/species: Pseudomonas putida
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
pyoverdin G4R: XDXXXGSXX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 42 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | G4R | 1 |
| 2 | entity_GA | 2 |
Entities:
Entity 1, G4R 9 residues - Formula weight is not available
| 1 | SIN | ASP | ORN | BHD | DAB | GLY | SER | ONH | CHR |
Entity 2, entity_GA - Ga - 69.723 Da.
| 1 | GA |
Samples:
sample_one: pyoverdin G4R 10 mM; entity_GA 10 mM; t-butanol, [U-2H], 2 % v/v
sample_two: pyoverdin G4R 10 mM; entity_GA 10 mM; t-butanol, [U-2H], 1 % v/v
sample_conditions_one: pH: 4.4; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H COSY | not available | not available | sample_conditions_one |
| 2D 1H-1H DQF-COSY | not available | not available | sample_conditions_one |
| 2D 1H-1H TOCSY | not available | not available | sample_conditions_one |
| 2D 1H-1H NOESY | not available | not available | sample_conditions_one |
| 2D 1H-1H ROESY | not available | not available | sample_conditions_one |
Software:
FELIX v2.1 - creation of plots, spectral processing
NMR spectrometers:
- Bruker AMX 500 MHz