BMRB Entry 4158

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4158

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H and 15N Nuclear Magnetic Resonance Chemical Shifts of the Cytotoxic Ribonuclease Alpha-sarcin

Deposition date:

1998-07-03

Original release date:

2000-03-08

Authors:

Rico, Manuel; Bruix, Marta; Santoro, Jorge; Campos-Olivas, Ramon; Perez-Canadillas, Jose

Citation:

Citation: Campos-Olivas, Ramon; Bruix, Marta; Santoro, Jorge; Martinez del Pozo, Alvaro; Lacadena, Javier; Gavilanes, Jose; Rico, Manuel. "1H and 15N Nuclear Magnetic Resonance Assignment and Secondary Structure of the Cytotoxic Ribonuclease Alpha-sarcin" Protein Sci. 5, 969-972 (1996).

Assembly members:

Assembly members:
Ribonuclease alpha-sarcin, polymer, 150 residues, 16981 Da.

Natural source:

Natural source: Common Name: Aspergillus giganteus Taxonomy ID: 5060 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Aspergillus giganteus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pINPGaS

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ribonuclease alpha-sarcin: AVTWTCLNDQKNPKTNKYET KRLLYNQNKAESNSHHAPLS DGKTGSSYPHWFTNGYDGDG KLPKGRTPIKFGKSDCDRPP KHSKDGNGKTDHYLLEFPTF PDGHDYKFDSKKPKENPGPA RVIYTYPNKVFCGIIAHTKE NQGELKLCSH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_one

Data type	Count
15N chemical shifts	154
1H chemical shifts	908

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	alpha sarcin	1

Entities:

Entity 1, alpha sarcin 150 residues - 16981 Da.

1	ALA	VAL	THR	TRP	THR	CYS	LEU	ASN	ASP	GLN
2	LYS	ASN	PRO	LYS	THR	ASN	LYS	TYR	GLU	THR
3	LYS	ARG	LEU	LEU	TYR	ASN	GLN	ASN	LYS	ALA
4	GLU	SER	ASN	SER	HIS	HIS	ALA	PRO	LEU	SER
5	ASP	GLY	LYS	THR	GLY	SER	SER	TYR	PRO	HIS
6	TRP	PHE	THR	ASN	GLY	TYR	ASP	GLY	ASP	GLY
7	LYS	LEU	PRO	LYS	GLY	ARG	THR	PRO	ILE	LYS
8	PHE	GLY	LYS	SER	ASP	CYS	ASP	ARG	PRO	PRO
9	LYS	HIS	SER	LYS	ASP	GLY	ASN	GLY	LYS	THR
10	ASP	HIS	TYR	LEU	LEU	GLU	PHE	PRO	THR	PHE
11	PRO	ASP	GLY	HIS	ASP	TYR	LYS	PHE	ASP	SER
12	LYS	LYS	PRO	LYS	GLU	ASN	PRO	GLY	PRO	ALA
13	ARG	VAL	ILE	TYR	THR	TYR	PRO	ASN	LYS	VAL
14	PHE	CYS	GLY	ILE	ILE	ALA	HIS	THR	LYS	GLU
15	ASN	GLN	GLY	GLU	LEU	LYS	LEU	CYS	SER	HIS

Samples:

sample_one: Ribonuclease alpha-sarcin, [U-15N], 1.5 mM

sample_conditions_one: pH: 4.0; temperature: 306 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	sample_one	not available	sample_conditions_one

Software:

No software information available

NMR spectrometers:

unknown unknown 0 MHz

PDB	1DE3
DBJ	BAA02863
EMBL	CAA37471 CAA43180
GB	AAB66603 AAB66605 ACA34736
SP	P00655
AlphaFold	P00655

1	ALA	VAL	THR	TRP	THR	CYS	LEU	ASN	ASP	GLN
2	LYS	ASN	PRO	LYS	THR	ASN	LYS	TYR	GLU	THR
3	LYS	ARG	LEU	LEU	TYR	ASN	GLN	ASN	LYS	ALA
4	GLU	SER	ASN	SER	HIS	HIS	ALA	PRO	LEU	SER
5	ASP	GLY	LYS	THR	GLY	SER	SER	TYR	PRO	HIS
6	TRP	PHE	THR	ASN	GLY	TYR	ASP	GLY	ASP	GLY
7	LYS	LEU	PRO	LYS	GLY	ARG	THR	PRO	ILE	LYS
8	PHE	GLY	LYS	SER	ASP	CYS	ASP	ARG	PRO	PRO
9	LYS	HIS	SER	LYS	ASP	GLY	ASN	GLY	LYS	THR
10	ASP	HIS	TYR	LEU	LEU	GLU	PHE	PRO	THR	PHE
11	PRO	ASP	GLY	HIS	ASP	TYR	LYS	PHE	ASP	SER
12	LYS	LYS	PRO	LYS	GLU	ASN	PRO	GLY	PRO	ALA
13	ARG	VAL	ILE	TYR	THR	TYR	PRO	ASN	LYS	VAL
14	PHE	CYS	GLY	ILE	ILE	ALA	HIS	THR	LYS	GLU
15	ASN	GLN	GLY	GLU	LEU	LYS	LEU	CYS	SER	HIS

1	ALA	VAL	THR	TRP	THR	CYS	LEU	ASN	ASP	GLN
2	LYS	ASN	PRO	LYS	THR	ASN	LYS	TYR	GLU	THR
3	LYS	ARG	LEU	LEU	TYR	ASN	GLN	ASN	LYS	ALA
4	GLU	SER	ASN	SER	HIS	HIS	ALA	PRO	LEU	SER
5	ASP	GLY	LYS	THR	GLY	SER	SER	TYR	PRO	HIS
6	TRP	PHE	THR	ASN	GLY	TYR	ASP	GLY	ASP	GLY
7	LYS	LEU	PRO	LYS	GLY	ARG	THR	PRO	ILE	LYS
8	PHE	GLY	LYS	SER	ASP	CYS	ASP	ARG	PRO	PRO
9	LYS	HIS	SER	LYS	ASP	GLY	ASN	GLY	LYS	THR
10	ASP	HIS	TYR	LEU	LEU	GLU	PHE	PRO	THR	PHE
11	PRO	ASP	GLY	HIS	ASP	TYR	LYS	PHE	ASP	SER
12	LYS	LYS	PRO	LYS	GLU	ASN	PRO	GLY	PRO	ALA
13	ARG	VAL	ILE	TYR	THR	TYR	PRO	ASN	LYS	VAL
14	PHE	CYS	GLY	ILE	ILE	ALA	HIS	THR	LYS	GLU
15	ASN	GLN	GLY	GLU	LEU	LYS	LEU	CYS	SER	HIS