BMRB Entry 4144
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4144
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Citation: Hodach, Meri; Elorante, Jyrki; Kostourou, Vassiliki; Wienzierl, Robert; Matthews, Stephen. "1H, 13C, 15N Sequential Assignment of the RNA Polymerase 'H' Subunit from
Methanococcus jannaschii" J Biomol. NMR ., .-. (1998).
Assembly members:
RPB5, polymer, 78 residues, Formula weight is not available
Natural source: Common Name: Methanococcus jannaschii Taxonomy ID: 2190 Superkingdom: Archaea Kingdom: Euryarchaeota Genus/species: Methanococcus jannaschii
Experimental source: Production method: recombinant technology Host organism: Methanoccocus jannaschii Vector: pGEX-2T
Entity Sequences (FASTA):
RPB5: MKVTDHILVPKHEIVPKEEV
EEILKRYNIKIQQLPKIYED
DPVIQEIGAKEGDVVRVIRK
SPTAGVSIAYRLVIKRII
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 219 |
| 15N chemical shifts | 68 |
| 1H chemical shifts | 151 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | rpb5 | 1 |
Entities:
Entity 1, rpb5 78 residues - Formula weight is not available
| 1 | MET | LYS | VAL | THR | ASP | HIS | ILE | LEU | VAL | PRO | ||||
| 2 | LYS | HIS | GLU | ILE | VAL | PRO | LYS | GLU | GLU | VAL | ||||
| 3 | GLU | GLU | ILE | LEU | LYS | ARG | TYR | ASN | ILE | LYS | ||||
| 4 | ILE | GLN | GLN | LEU | PRO | LYS | ILE | TYR | GLU | ASP | ||||
| 5 | ASP | PRO | VAL | ILE | GLN | GLU | ILE | GLY | ALA | LYS | ||||
| 6 | GLU | GLY | ASP | VAL | VAL | ARG | VAL | ILE | ARG | LYS | ||||
| 7 | SER | PRO | THR | ALA | GLY | VAL | SER | ILE | ALA | TYR | ||||
| 8 | ARG | LEU | VAL | ILE | LYS | ARG | ILE | ILE |
Samples:
sample_one: RPB5, [U-13C;U-15N], 1.0 mM; H2O 90%; D2O 10%
sample_conditions_one: pH: 5.0; temperature: 302 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Bruker DRX-500 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks