BMRB Entry 4140

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4140

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Title:
Sequence-specific 1H, 13C and 15N Assignment of the EH1 Domain of Mouse Eps15
Deposition date:
1998-05-06
Original release date:
1999-02-03
Authors:
Whitehead, Brian; Tessari, Tessari; Versteeg, Henri; van Delft, Sanne; van Bergen en Henegouwen, Paul; Vuister, Geerten
Citation:

Citation: Whitehead, Brian; Tessari, Tessari; Versteeg, Henri; van Delft, Sanne; van Bergen en Henegouwen, Paul; Vuister, Geerten. "Sequence-specific 1H, 13C and 15N Assignment of the EH1 Domain of Mouse Eps15"  J. Biomol. NMR 12, 465-466 (1998).

Assembly members:

Assembly members:
EH1 domain of mouse Eps15, polymer, 120 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGex-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EH1 domain of mouse Eps15: MAAAAQLSLTQLSSGNPVYE KYYRQVEAGNTGRVLALDAA AFLKKSGLPDLILGKIWDLA DTDGKGVLSKQEFFVALRLV ACAQNGLEVSLSSLSLAVPP PRFHDSSSPLLTSGPSVAEL

Data sets:
Data typeCount
13C chemical shifts501
15N chemical shifts124
1H chemical shifts774

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Eps15-EH11

Entities:

Entity 1, Eps15-EH1 120 residues - Formula weight is not available

1   METALAALAALAALAGLNLEUSERLEUTHR
2   GLNLEUSERSERGLYASNPROVALTYRGLU
3   LYSTYRTYRARGGLNVALGLUALAGLYASN
4   THRGLYARGVALLEUALALEUASPALAALA
5   ALAPHELEULYSLYSSERGLYLEUPROASP
6   LEUILELEUGLYLYSILETRPASPLEUALA
7   ASPTHRASPGLYLYSGLYVALLEUSERLYS
8   GLNGLUPHEPHEVALALALEUARGLEUVAL
9   ALACYSALAGLNASNGLYLEUGLUVALSER
10   LEUSERSERLEUSERLEUALAVALPROPRO
11   PROARGPHEHISASPSERSERSERPROLEU
12   LEUTHRSERGLYPROSERVALALAGLULEU

Samples:

sample_one: EH1 domain of mouse Eps15, [U-15N], 1.0 mM; potassium phosphate 100 mM; sodium chloride 100 mM; calcium chloride 10 mM; H2O 90%; D2O 10%

sample_two: EH1 domain of mouse Eps15, [U-15N;U-13C], 1.0 mM; potassium phosphate 100 mM; sodium chloride 100 mM; calcium chloride 10 mM; H2O 90%; D2O 10%

sample_conditions_one: pH: 5.2; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian Unity Inova 500 MHz
  • Varian Unity Inova 750 MHz

Related Database Links:

BMRB 4491
PDB

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