BMRB Entry 4134

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4134

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Title:
NMR Assignments for Denatured LysN
Deposition date:
1998-04-16
Original release date:
1999-06-17
Authors:
Alexandrescu, Andrei; Jaravine, Viktor; Lamour, Francois
Citation:

Citation: Alexandrescu, Andrei; Jaravine, Viktor; Lamour, Francois. "NMR Assignments for denatured LysN"  (1999).

Assembly members:

Assembly members:
lysl-tRNA_synthetase, polymer, 120 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
lysl-tRNA_synthetase: AEQGIAFPNDFRRDHTSDQL HAEFDGKENEELEALNIEVA VAGRMMTRRIMGKASFVTLQ DVGGRIQLYVARDDLPEGVY NEQFKKWDLGDILGAKGKLF KTKTGELSIHCTELRLLTKA

Data sets:
Data typeCount
13C chemical shifts219
15N chemical shifts115
1H chemical shifts420

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1den-LysN1

Entities:

Entity 1, den-LysN 120 residues - Formula weight is not available

1   ALAGLUGLNGLYILEALAPHEPROASNASP
2   PHEARGARGASPHISTHRSERASPGLNLEU
3   HISALAGLUPHEASPGLYLYSGLUASNGLU
4   GLULEUGLUALALEUASNILEGLUVALALA
5   VALALAGLYARGMETMETTHRARGARGILE
6   METGLYLYSALASERPHEVALTHRLEUGLN
7   ASPVALGLYGLYARGILEGLNLEUTYRVAL
8   ALAARGASPASPLEUPROGLUGLYVALTYR
9   ASNGLUGLNPHELYSLYSTRPASPLEUGLY
10   ASPILELEUGLYALALYSGLYLYSLEUPHE
11   LYSTHRLYSTHRGLYGLULEUSERILEHIS
12   CYSTHRGLULEUARGLEULEUTHRLYSALA

Samples:

sample_one: lysl-tRNA_synthetase, [U-15N; U-13C], 1.5 mM; urea 3 M; H2O 90%; D2O 10%

sample_two: lysl-tRNA_synthetase, [U-15N], 1 mM; urea 3 M; H2O 90%; D2O 10%

sample_conditions_one: pH: 2.8 na; pressure: 1 atm; temperature: 284 K

Experiments:

NameSampleSample stateSample conditions
NMR spectral acquisition parameterssample_twonot availablesample_conditions_one
t3 H 599.9094119 146ms . 7000Hz No 1024 complex .sample_twonot availablesample_conditions_one
t2 C 150.8529083 7.7ms . 8300Hz No 64 complex .sample_twonot availablesample_conditions_one
t1 N 60.7952139 21ms . 1540Hz No 32 complex .sample_twonot availablesample_conditions_one
NMR spectral processing parameterssample_twonot availablesample_conditions_one
D1 H sine-bell 1024 90 Zero-fill 2048sample_twonot availablesample_conditions_one
D2 C squared-sine-bell 64 70 Zero-fill 128sample_twonot availablesample_conditions_one
D3 N linear predict 32 to 48 squaredsine-bell 48 70 Zero-fill 64sample_twonot availablesample_conditions_one
46 ms mix timesample_onenot availablesample_conditions_one
NMR spectral acquisition parameterssample_onenot availablesample_conditions_one
t3 H 600.1328206MHz 85ms 46ms 6009.615Hz No 512 complex .sample_onenot availablesample_conditions_one
t2 N 60.8178387MHz 21ms . 1521Hz No 32 complex .sample_onenot availablesample_conditions_one
t1 H 600.1328206MHz 21ms . 6009.615Hz No 128 complex .sample_onenot availablesample_conditions_one
NMR spectral processing parameterssample_onenot availablesample_conditions_one
3D [1H-15N] TOCSY-HSQCsample_onenot availablesample_conditions_one
D1 H gm -10 0.1 Zero-fill 1024sample_onenot availablesample_conditions_one
D2 N lpl 32 to 48 gm -13 0.1 Zero-fill 64sample_onenot availablesample_conditions_one
D3 H gm -13 0.1 zf 256sample_onenot availablesample_conditions_one
200 ms mix timesample_onenot availablesample_conditions_one
t3 H 599.9094183MHz 146ms 200ms 7000Hz No 1024 complex .sample_onenot availablesample_conditions_one
t2 H 599.9094183MHz 18ms . 7000Hz No 128 complex .sample_onenot availablesample_conditions_one
t1 N 60.7951139MHz 21ms . 1540Hz No 32 complex .sample_onenot availablesample_conditions_one
3D [1H-15N] NOESY-HSQCsample_onenot availablesample_conditions_one
D1 H gm -5 0.1 Zero-fill 2048sample_onenot availablesample_conditions_one
D2 H gm -7 0.1 Zero-fill 256sample_onenot availablesample_conditions_one
D3 N lpl 32 to 48 gm -15 0.1 Zero-fill 64sample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Varian Unity plus 600 MHz

Related Database Links:

PDB
DBJ BAB37185 BAE76955 BAG78677 BAI27164 BAI32202
EMBL CAP77327 CAQ33202 CAQ90319 CAQ99824 CAR04406
GB AAA23959 AAA83071 AAC75928 AAG58018 AAN44362
REF NP_311789 NP_417366 NP_708655 WP_000003059 WP_000003060
SP B2U0Q7 P0A8N3 P0A8N4 Q0T106 Q31WF2
AlphaFold B2U0Q7 P0A8N3 P0A8N4 Q0T106 Q31WF2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks