BMRB Entry 4132

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4132

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone Resonance Assignments of Human UBC9
Deposition date:
1998-04-07
Original release date:
1999-05-25
Authors:
Liu, Qin; Shen, Binghui; Chen, David; Chen, Yuan
Citation:

Citation: Liu, Qin; Shen, Binghui; Chen, David; Chen, Yuan. "Backbone Resonance Assignments of Human UBC9"  J. Biomol. NMR 13, 89-90 (1999).

Assembly members:

Assembly members:
human ubiquitin-conjugating enzyme, polymer, 166 residues, 18874 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: not available   Superkingdom: eucaryote   Kingdom: Animalia   Genus/species: homo sapien

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human ubiquitin-conjugating enzyme: MGHHHHHHMSGIALSRLAQE RKAWRKDHPFGFVAVPTKNP DGTMNLMNWECAIPGKKGTP WEGGLFKLRMLFKDDYPSSP PKCKFEPPLFHPNVYPSGTV CLSILEEDKDWRPAITIKQI LLGIQELLNEPNIQDPAQAE AYTIYCQNRVEYEKRVRAQA KKFAPS

Data sets:
Data typeCount
1H chemical shifts586
13C chemical shifts308
15N chemical shifts142

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hUBC91

Entities:

Entity 1, hUBC9 166 residues - 18874 Da.

1   METGLYHISHISHISHISHISHISMETSER
2   GLYILEALALEUSERARGLEUALAGLNGLU
3   ARGLYSALATRPARGLYSASPHISPROPHE
4   GLYPHEVALALAVALPROTHRLYSASNPRO
5   ASPGLYTHRMETASNLEUMETASNTRPGLU
6   CYSALAILEPROGLYLYSLYSGLYTHRPRO
7   TRPGLUGLYGLYLEUPHELYSLEUARGMET
8   LEUPHELYSASPASPTYRPROSERSERPRO
9   PROLYSCYSLYSPHEGLUPROPROLEUPHE
10   HISPROASNVALTYRPROSERGLYTHRVAL
11   CYSLEUSERILELEUGLUGLUASPLYSASP
12   TRPARGPROALAILETHRILELYSGLNILE
13   LEULEUGLYILEGLNGLULEULEUASNGLU
14   PROASNILEGLNASPPROALAGLNALAGLU
15   ALATYRTHRILETYRCYSGLNASNARGVAL
16   GLUTYRGLULYSARGVALARGALAGLNALA
17   LYSLYSPHEALAPROSER

Samples:

sample_one: human ubiquitin-conjugating enzyme, [U-15N], 0.7 mM; sodium phosphate 100 mM; H2O 90%; D2O 10%

sample_two: human ubiquitin-conjugating enzyme, [U-13C; U-15N], 0.7 mM; sodium phosphate 100 mM; H2O 90%; D2O 10%

sample_three: human ubiquitin-conjugating enzyme, [U-13C; U-15N; U-70% 2H], 0.7 mM; sodium phosphate 100 mM; H2O 90%; D2O 10%

sample_conditions_one: pH: 6.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
DBJ BAA08091 BAB22599 BAB23783 BAB27487 BAB28140
EMBL CAA05359 CAA65287 CAA66188 CAA68072 CAJ81434
GB AAA86662 AAB02181 AAB02182 AAB09410 AAB18790
REF NP_001016408 NP_001080758 NP_001092842 NP_001171080 NP_001171081
SP P63279 P63280 P63281 P63282 P63283
TPG DAA15584
AlphaFold P63279 P63280 P63281 P63282 P63283

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks