BMRB Entry 4131
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4131
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NMR-STAR v3 text file.
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Citation: Dangi, Bindi; Sarma, Siddhartha; Yan, Chunhua; Banville, Debra; Guiles, Ronald. "The Origin of Difference in the Physical Properties of the Equilibrium Forms of
Cytochrome b5 Revealed Through High Resolution NMR Structures and Backbone
Dynamic Analyses" Biochemistry 37, 8289-8302 (1998).
Assembly members:
cytochrome b5, polymer, 94 residues, Formula weight is not available
HEME C, non-polymer, 618.503 Da.
Natural source: Common Name: rat Taxonomy ID: 10117 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus rattus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3c
Entity Sequences (FASTA):
cytochrome b5: DKDVKYYTLEEIQKHKDSKS
TWVILHHKVYDLTKFLEEHP
GGEEVLREQAGGDATENFED
VGHSTDARELSKTYIIGELH
PDDRSKIAKPSETL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 514 |
| 15N chemical shifts | 90 |
| 13C chemical shifts | 297 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cytb5_B | 1 |
| 2 | heme | 2 |
Entities:
Entity 1, cytb5_B 94 residues - Formula weight is not available
| 1 | ASP | LYS | ASP | VAL | LYS | TYR | TYR | THR | LEU | GLU | ||||
| 2 | GLU | ILE | GLN | LYS | HIS | LYS | ASP | SER | LYS | SER | ||||
| 3 | THR | TRP | VAL | ILE | LEU | HIS | HIS | LYS | VAL | TYR | ||||
| 4 | ASP | LEU | THR | LYS | PHE | LEU | GLU | GLU | HIS | PRO | ||||
| 5 | GLY | GLY | GLU | GLU | VAL | LEU | ARG | GLU | GLN | ALA | ||||
| 6 | GLY | GLY | ASP | ALA | THR | GLU | ASN | PHE | GLU | ASP | ||||
| 7 | VAL | GLY | HIS | SER | THR | ASP | ALA | ARG | GLU | LEU | ||||
| 8 | SER | LYS | THR | TYR | ILE | ILE | GLY | GLU | LEU | HIS | ||||
| 9 | PRO | ASP | ASP | ARG | SER | LYS | ILE | ALA | LYS | PRO | ||||
| 10 | SER | GLU | THR | LEU |
Entity 2, heme - C34 H34 Fe N4 O4 - 618.503 Da.
| 1 | HEC |
Samples:
sample_one: rat ferrocytochrome b5 B form, [U-15N,; U-13C], 4 mM; phosphate 100 mM
sample_conditions_one: ionic strength: 100 .; pH: 7.0; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_one | not available | sample_conditions_one |
Software:
No software information available
NMR spectrometers:
- Bruker DMX600 600 MHz
Related Database Links:
| BMRB | 4130 |
| PDB | |
| DBJ | BAA02492 BAB22093 BAB28714 BAE20982 |
| GB | AAA67175 AAA72420 AAA72421 AAA72557 AAA99718 |
| REF | NP_001268328 NP_071581 NP_080073 XP_003512427 XP_005356388 |
| SP | P00173 P56395 |
| AlphaFold | P00173 P56395 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks