BMRB Entry 4125

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4125

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The NMR Structure of 31mer RNA Domain of Escherichia Coli RNase P RNA Using Its Non-uniformly Deuterium Labelled Counterpart [the 'NMR-window' concept]

Deposition date:

1998-03-17

Original release date:

1998-04-07

Authors:

Glemarec, C.; Kufel, J.; Foldesi, A.; Maltseva, T.; Sandstrom, A.; Kirsebom, L.; Chattopadhyaya, J.

Citation:

Citation: Glemarec, C.; Kufel, J.; Foldesi, A.; Maltseva, T.; Sandstrom, A.; Kirsebom, L.; Chattopadhyaya, J.. "The NMR Structure of 31mer RNA Domain of Escherichia Coli RNase P RNA Using Its Non-uniformly Deuterium Labelled Counterpart [the 'NMR-window' concept]" Nucleic Acids Res. 24, 2022-2035 (1996).

Assembly members:

Assembly members:
31mer_RNA_1, polymer, 31 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
31mer_RNA_1: GGCCAAAUAGGGGCUUCGGC CCGGGUAGGCU

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment_one

Data type	Count
1H chemical shifts	192

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	31mer_RNA_1	1

Entities:

Entity 1, 31mer_RNA_1 31 residues - Formula weight is not available

1

G

C

A

U

A

G

2

G

C

U

C

G

C

3

C

G

U

A

G

C

4

U

Samples:

sample_one: 31mer_RNA_1, [U-99%-2H]-C, 1 mM; NaHPO4 10 mM; NaCl 50 mM; EDTA 0.1 mM

sample_two: 31mer_RNA_1 1 mM; NaHPO4 10 mM; NaCl 50 mM; EDTA 0.1 mM

sample_conditions_one: pH: 6.2; temperature: 299 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	not available	not available	not available

Software:

No software information available

NMR spectrometers:

unknown unknown 0 MHz