BMRB Entry 4122

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4122

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Title:
Solution Structure of the Human Hck SH3 Domain and Identification of its Ligand Binding Site
Deposition date:
1998-03-09
Original release date:
1999-02-02
Authors:
Horita, David; Baldisseri, Donna; Zhang, Weixing; Altieri, Amanda; Smithgall, Thomas; Gmeiner, William; Byrd, Andrew
Citation:

Citation: Horita, David; Baldisseri, Donna; Zhang, Weixing; Altieri, Amanda; Smithgall, Thomas; Gmeiner, William; Byrd, Andrew. "Solution Structure of the Human Hck SH3 Domain and Identification of its Ligand Binding Site"  J. Mol. Biol. 278, 253-265 (1998).

Assembly members:

Assembly members:
Hematopoietic cell kinase, polymer, 72 residues, 8229 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET14b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hematopoietic cell kinase: GIREAGSEDIIVVALYDYEA IHHEDLSFQKGDQMVVLEES GEWWKARSLATRKEGYIPSN YVARVDSLETEE

Data sets:
Data typeCount
13C chemical shifts307
15N chemical shifts73
1H chemical shifts486

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hck_SH1

Entities:

Entity 1, Hck_SH 72 residues - 8229 Da.

1   GLYILEARGGLUALAGLYSERGLUASPILE
2   ILEVALVALALALEUTYRASPTYRGLUALA
3   ILEHISHISGLUASPLEUSERPHEGLNLYS
4   GLYASPGLNMETVALVALLEUGLUGLUSER
5   GLYGLUTRPTRPLYSALAARGSERLEUALA
6   THRARGLYSGLUGLYTYRILEPROSERASN
7   TYRVALALAARGVALASPSERLEUGLUTHR
8   GLUGLU

Samples:

sample_one: Hematopoietic cell kinase, [U-13C; U-15N], 1.0 mM

sample_conditions_one: pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 17740 17819
PDB
DBJ BAB15482 BAF82585 BAF83617 BAG60878
EMBL CAC44031
GB AAA52643 AAA52644 AAH14435 AAH94847 AAI08931
REF NP_001165600 NP_001165601 NP_001165602 NP_001165603 NP_001165604
SP P08631 Q95M30
AlphaFold P08631 Q95M30

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks