BMRB Entry 4121

Name: 1H, 13C and 15N Assignment of the Isl-1 Homeodomain
Published: 1998-10-09

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4121

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N Assignment of the Isl-1 Homeodomain

Deposition date:

1998-03-09

Original release date:

1998-10-09

Authors:

Ippel, Johannes; Larsson, Goran; Behravan, Gity; Lundqvist, Martin; Lycksell, Per-Olof; Schleucher, Juergen; Zdunek, Janusz; Wijmenga, Sybren

Citation:

Citation: Ippel, Johannes; Larsson, Goran; Behravan, Gity; Lundqvist, Martin; Lycksell, Per-Olof; Schleucher, Juergen; Zdunek, Janusz; Wijmenga, Sybren. "1H, 13C and 15N Assignment of the Isl-1 Homeodomain" J. Biomol. NMR 12, 357-359 (1998).

Assembly members:

Assembly members:
Isl-1 homeodomain, polymer, 66 residues, 7860 Da.

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10117 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus rattus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Isl-1 homeodomain: MKTTRVRTVLNEKQLHTLRT CYAANPRPDALMKEQLVEMT GLSPRVIRVWFQNKRCKDKK RSIMMK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_one
coupling_constants
- coupling_constants

Data type	Count
13C chemical shifts	162
15N chemical shifts	68
1H chemical shifts	474
coupling constants	60

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Isl-1-HD	1

Entities:

Entity 1, Isl-1-HD 66 residues - 7860 Da.

1

MET

LYS

THR

ARG

VAL

ARG

THR

VAL

LEU

2

ASN

GLU

LYS

GLN

LEU

HIS

THR

LEU

ARG

THR

3

CYS

TYR

ALA

ASN

PRO

ARG

PRO

ASP

ALA

4

LEU

MET

LYS

GLU

GLN

LEU

VAL

GLU

MET

THR

5

GLY

LEU

SER

PRO

ARG

VAL

ILE

ARG

VAL

TRP

6

PHE

GLN

ASN

LYS

ARG

CYS

LYS

ASP

LYS

7

ARG

SER

ILE

MET

LYS

Samples:

sample_one: Isl-1 homeodomain 0.8 mM; DTT 10 mM; NaCl 25 mM; Na2HPO4/NaH2PO4 25 mM; D2O 10%; H2O 90%

sample_two: Isl-1 homeodomain, [U-15N], 0.45 mM; DTT 10 mM; NaCl 25 mM; Na2HPO4/NaH2PO4 25 mM; D2O 10%; H2O 90%

sample_three: Isl-1 homeodomain, [U-15N;U-13C], 1.8 mM; DTT 10 mM; NaCl 25 mM; Na2HPO4/NaH2PO4 25 mM; D2O 10%; H2O 90%

sample_conditions_one: pH: 6.0; temperature: 281 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

No software information available

NMR spectrometers:

Bruker DRX600 600 MHz

PDB	1BW5
DBJ	BAA04670 BAA07485 BAB29088 BAC41153 BAC57891
EMBL	CAA37349 CAB38446 CAI11499 CDQ88523 CDQ89879
GB	AAA62161 AAA62171 AAA62172 AAA80274 AAA80275
PIR	I67418
PRF	1608207A
REF	NP_001092600 NP_001104188 NP_001159513 NP_002193 NP_059035
SP	P50211 P50480 P53405 P53407 P53408
TPG	DAA17547 DAA17902
AlphaFold	P50211 P50480 P53405 P53407 P53408