BMRB Entry 4117
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4117
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Citation: Perez, Janice; Kriek, Jan; Dijk, Jan; Moller, Wim; Siegal, Gregg; Hard, Karl; Kalverda, Arnout; Canters, Gerard. "1H, 15N and 13C Chemical Shift Assignment of the Guanine Nucleotide
Exchange Domain of Human Elongation Factor-one Beta" J. Biomol. NMR 12, 467-468 (1998).
Assembly members:
human Elongation Factor-1beta, polymer, 91 residues, 10079 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a
Entity Sequences (FASTA):
human Elongation Factor-1beta: MLVAKSSILLDVKPWDDETD
MAKLEECVRSIQADGLVWGS
SKLVPVGYGIKKLQIQCVVE
DDKVGTDMLEEQITAFEDYV
QSMDVAAFNKI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 370 |
| 15N chemical shifts | 95 |
| 1H chemical shifts | 642 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hEF-1beta | 1 |
Entities:
Entity 1, hEF-1beta 91 residues - 10079 Da.
| 1 | MET | LEU | VAL | ALA | LYS | SER | SER | ILE | LEU | LEU | ||||
| 2 | ASP | VAL | LYS | PRO | TRP | ASP | ASP | GLU | THR | ASP | ||||
| 3 | MET | ALA | LYS | LEU | GLU | GLU | CYS | VAL | ARG | SER | ||||
| 4 | ILE | GLN | ALA | ASP | GLY | LEU | VAL | TRP | GLY | SER | ||||
| 5 | SER | LYS | LEU | VAL | PRO | VAL | GLY | TYR | GLY | ILE | ||||
| 6 | LYS | LYS | LEU | GLN | ILE | GLN | CYS | VAL | VAL | GLU | ||||
| 7 | ASP | ASP | LYS | VAL | GLY | THR | ASP | MET | LEU | GLU | ||||
| 8 | GLU | GLN | ILE | THR | ALA | PHE | GLU | ASP | TYR | VAL | ||||
| 9 | GLN | SER | MET | ASP | VAL | ALA | ALA | PHE | ASN | LYS | ||||
| 10 | ILE |
Samples:
sample_one: human Elongation Factor-1beta, [U-13C; U-15N], 1 mM; NaCl 100 mM; NaPO4 10 mM; DTT 1 mM; NaN3 0.02%; H2O 95%; D2O 5%
sample_conditions_one: ionic strength: 0.1 M; pH: 6.9; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
| PDB | |
| DBJ | BAB28447 BAC25661 BAE40151 BAE87819 BAE88158 |
| EMBL | CAA43019 CAA43063 CAA52741 CAG32662 CAG33106 |
| GB | AAC13264 AAD16874 AAH00211 AAH03899 AAH04931 |
| REF | NP_001014936 NP_001032752 NP_001075868 NP_001102269 NP_001230453 |
| SP | O70251 P24534 P34826 Q5E983 Q9YGQ1 |
| TPG | DAA32476 |
| AlphaFold | O70251 P24534 P34826 Q5E983 Q9YGQ1 |
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