BMRB Entry 4115

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4115

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Assignment of 1H, 13C and 15N Signals of the Inhibitor Protein Im9 Bound to the DNase Domain of Colicin E9

Deposition date:

1998-03-04

Original release date:

2006-10-27

Authors:

Boetzel, Ruth; Czisch, Michael; MacDonald, Colin; Kaptein, Robert; Hemmings, Andrew; James, Richard; Kleanthous, Colin; Moore, Geoffrey

Citation:

Citation: Boetzel, Ruth; Czisch, Michael; MacDonald, Colin; Kaptein, Robert; Hemmings, Andrew; James, Richard; Kleanthous, Colin; Moore, Geoffrey. "Assignment of 1H, 13C and 15N Signals of the Inhibitor Protein Im9 Bound to the DNase Domain of Colicin E9" J. Biomol. NMR 12, 567-568 (1998).

Assembly members:

Assembly members:
IM9, polymer, 86 residues, Formula weight is not available
E9, polymer, . residues, Formula weight is not available

Natural source:

Natural source: Common Name: E.coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
IM9: MELKHSISDYTEAEFLQLVT TICNADTSSEEELVKLVTHF EEMTEHPSGSDLIYYPKEGD DDSPSGIVNTVKQWRAANGK SGFKQG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_one

Data type	Count
13C chemical shifts	254
15N chemical shifts	81
1H chemical shifts	529

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	IM9	1
2	E9	2

Entities:

Entity 1, IM9 86 residues - Formula weight is not available

1

MET

GLU

LEU

LYS

HIS

SER

ILE

SER

ASP

TYR

2

THR

GLU

ALA

GLU

PHE

LEU

GLN

LEU

VAL

THR

3

THR

ILE

CYS

ASN

ALA

ASP

THR

SER

GLU

4

GLU

LEU

VAL

LYS

LEU

VAL

THR

HIS

PHE

5

GLU

MET

THR

GLU

HIS

PRO

SER

GLY

SER

6

ASP

LEU

ILE

TYR

PRO

LYS

GLU

GLY

ASP

7

ASP

SER

PRO

SER

GLY

ILE

VAL

ASN

THR

8

VAL

LYS

GLN

TRP

ARG

ALA

ASN

GLY

LYS

9

SER

GLY

PHE

LYS

GLN

GLY

Entity 2, E9 - Formula weight is not available

Samples:

sample: IM9, [U-100% 15N; U-100% 13C], 1.2 mM; E9, [U-100% 15N; U-100% 13C], 1.3 mM; H2O 90%; D2O 10%

sample_conditions: pH: 6.2 na; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	sample	not available	sample_conditions

Software:

No software information available

NMR spectrometers:

. . 500 MHz
. . 600 MHz

BMRB	4116
PDB	1BXI 1E0H 1EMV 1FR2 1IMP 1IMQ 2GYK 2GZE 2GZF 2GZG 2GZI 2GZJ 2K5X 2VLN 2VLO 2VLP 2VLQ
EMBL	CAA33863
GB	AAA23076 ACM07431
REF	WP_012644887 YP_002533538
SP	P13479
AlphaFold	P13479