BMRB Entry 4110

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4110

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, 15N Resonance Assignment of the 20 KDa Double Stranded RNA Binding Domain of PKR

Deposition date:

1998-02-19

Original release date:

1998-10-14

Authors:

Nanduri, Sambasivarao; Carpick, Bruce; Yang, Yan; Williams, Bryan; Qin, Jun

Citation:

Citation: Nanduri, Sambasivarao; Carpick, Bruce; Yang, Yan; Williams, Bryan; Qin, Jun. "1H, 13C, 15N Resonance Assignment of the 20 KDa Double Stranded RNA Binding Domain of PKR" J. Biomol. NMR 12, 349-351 (1998).

Assembly members:

Assembly members:
double stranded RNA binding domain of PKR, polymer, 179 residues, 20000 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
double stranded RNA binding domain of PKR: GSHMEMAGDLSAGFFMEELN TRYQKQGVVLKYQELPNSGP PHDRRFTFQVIIDGREFPEG EGRSKKEAKNAAAKLAVEIL NKEKKAVSPLLLTTTNSSEG LSMGNYIGLINRIAQKKRLT VNYEQCASGVHGPEGFHYKC KMGQKEYSIGTGSTKQEAKQ LAAKLAYLQILSEETGSGC

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_one

Data type	Count
13C chemical shifts	577
15N chemical shifts	176
1H chemical shifts	1183

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	dsRBD-PKR	1

Entities:

Entity 1, dsRBD-PKR 179 residues - 20000 Da.

1

GLY

SER

HIS

MET

GLU

MET

ALA

GLY

ASP

LEU

2

SER

ALA

GLY

PHE

MET

GLU

LEU

ASN

3

THR

ARG

TYR

GLN

LYS

GLN

GLY

VAL

LEU

4

LYS

TYR

GLN

GLU

LEU

PRO

ASN

SER

GLY

PRO

5

PRO

HIS

ASP

ARG

PHE

THR

PHE

GLN

VAL

6

ILE

ASP

GLY

ARG

GLU

PHE

PRO

GLU

GLY

7

GLU

GLY

ARG

SER

LYS

GLU

ALA

LYS

ASN

8

ALA

LYS

LEU

ALA

VAL

GLU

ILE

LEU

9

ASN

LYS

GLU

LYS

ALA

VAL

SER

PRO

LEU

10

LEU

THR

ASN

SER

GLU

GLY

11

LEU

SER

MET

GLY

ASN

TYR

ILE

GLY

LEU

ILE

12

ASN

ARG

ILE

ALA

GLN

LYS

ARG

LEU

THR

13

VAL

ASN

TYR

GLU

GLN

CYS

ALA

SER

GLY

VAL

14

HIS

GLY

PRO

GLU

GLY

PHE

HIS

TYR

LYS

CYS

15

LYS

MET

GLY

GLN

LYS

GLU

TYR

SER

ILE

GLY

16

THR

GLY

SER

THR

LYS

GLN

GLU

ALA

LYS

GLN

17

LEU

ALA

LYS

LEU

ALA

TYR

LEU

GLN

ILE

18

LEU

SER

GLU

THR

GLY

SER

GLY

CYS

Samples:

sample_one: double stranded RNA binding domain of PKR, [U-15N], .9 mM; NaCl 100 mM; sodium phosphate 20 mM; DTT 1 mM; H2O 93%; D2O 7%

sample_two: double stranded RNA binding domain of PKR, [U-15N;U-13C], .9 mM; NaCl 100 mM; sodium phosphate 20 mM; DTT 1 mM; H2O 93%; D2O 7%

sample_conditions_one: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	not available	not available	sample_conditions_one

Software:

NMRPipe - used for all data processing

PIPP - analysis of processed spectra

NMR spectrometers:

Varian Inova 500 MHz

PDB	1QU6
DBJ	BAF83344 BAG36554
GB	AAA18253 AAA36409 AAC50768 AAF13156 AAH07769
REF	NP_001129123 NP_001129124 NP_001138509 NP_001266110 NP_001266614
SP	P19525
AlphaFold	P19525