BMRB Entry 4107

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4107

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Title:
Acid Denatured Cold Shock Protein A (CspA)
Deposition date:
1998-02-16
Original release date:
1998-03-04
Authors:
Alexandrescu, Andrei; Rathgeb-Szabo, K.
Citation:

Citation: Alexandrescu, Andrei; Rathgeb-Szabo, K.. "NMR Assignments for Acid-Denatured Cold Shock Protein A"  J. Biomol. NMR 11, 461-462 (1998).

Assembly members:

Assembly members:
Cold shock protein A, polymer, 70 residues, 7400 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11-cspA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cold shock protein A: MSGKMTGIVKWFNADKGFGF ITPDDGSKDVFVHFSAIQND GYKSLDEGQKVSFTIESGAK GPAAGNVTSL

Data sets:
Data typeCount
13C chemical shifts124
15N chemical shifts66
1H chemical shifts270

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CspA1

Entities:

Entity 1, CspA 70 residues - 7400 Da.

1   METSERGLYLYSMETTHRGLYILEVALLYS
2   TRPPHEASNALAASPLYSGLYPHEGLYPHE
3   ILETHRPROASPASPGLYSERLYSASPVAL
4   PHEVALHISPHESERALAILEGLNASNASP
5   GLYTYRLYSSERLEUASPGLUGLYGLNLYS
6   VALSERPHETHRILEGLUSERGLYALALYS
7   GLYPROALAALAGLYASNVALTHRSERLEU

Samples:

sample_one: Cold shock protein A, [U-15N], 1 mM

sample_two: Cold shock protein A, [U-15N;U-13C], 2 mM

sample_conditions_one: pH: 2.0; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian Unity+600 600 MHz

Related Database Links:

BMRB 4108 4296
PDB
DBJ BAB37864 BAE77739 BAG79354 BAH65698 BAI28199
EMBL CAD07979 CAP78016 CAQ33874 CAQ91030 CAR00519
GB AAA23617 AAB18533 AAB66357 AAB69447 AAC06036
PIR AG0981
REF NP_312468 NP_418012 NP_458276 NP_462550 NP_709333
SP P0A9X9 P0A9Y0 P0A9Y1 P0A9Y2 P0A9Y3
AlphaFold P0A9X9 P0A9Y0 P0A9Y1 P0A9Y2 P0A9Y3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks