BMRB Entry 4103
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4103
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Citation: Fernandez, Cesar; Szyperski, Thomas; Ono, Akira; Iwai, Hideo; Tate, Shin-ichi; Kainosho, Masatsune; Wuthrich, Kurt. "NMR with 13C,15N-doubly-labeled DNA: the Antennapedia Homeodomain
Complex with a 14mer DNA Duplex" J. Biomol. NMR ., .-. (1998).
Assembly members:
BS2_strand_A, polymer, 14 residues, Formula weight is not available
BS2_strand_B, polymer, 14 residues, Formula weight is not available
Natural source: Common Name: fruit fly Taxonomy ID: not available Superkingdom: Eukaryota Kingdom: Animalia Genus/species: Drosophila melanogaster
Experimental source: Production method: Chemical synthesis
Entity Sequences (FASTA):
BS2_strand_A: GAAAGCCATTAGAG
BS2_strand_B: CTCTAATGGCTTTC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 188 |
| 15N chemical shifts | 22 |
| 1H chemical shifts | 258 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BS2_strand_A | 1 |
| 2 | BS2_strand_B | 2 |
Entities:
Entity 1, BS2_strand_A 14 residues - Formula weight is not available
| 1 | DG | DA | DA | DA | DG | DC | DC | DA | DT | DT | ||||
| 2 | DA | DG | DA | DG |
Entity 2, BS2_strand_B 14 residues - Formula weight is not available
| 1 | DC | DT | DC | DT | DA | DA | DT | DG | DG | DC | ||||
| 2 | DT | DT | DT | DC |
Samples:
sample_one: 14mer DNA duplex containing the operator sequence BS2, [U-13C;U-15N], 1.0 mM; KPO4 50 mM; KCL 20 mM; D2O 10%; H2O 90%
sample_conditions_one: pH: 6.0; temperature: 309 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_one | not available | sample_conditions_one |
Software:
No software information available
NMR spectrometers:
- Bruker DRX500 500 MHz
- Bruker DRX600 600 MHz
- Varian Unity Plus Unity Plus 750 MHz