BMRB Entry 4085

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4085

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Resonance Assignments and Solution Structure of the Second RNA-Binding Domain of Sex-lethal Determined by Multidimensional Heteronuclear Magnetic Resonance

Deposition date:

1997-12-23

Original release date:

2001-02-16

Authors:

Lee, Andrew; Kanaar, Roland; Rio, Donald; Wemmer, David

Citation:

Citation: Lee, Andrew; Kanaar, Roland; Rio, Donald; Wemmer, David. "Resonance Assignments and Solution Structure of the Second RNA-Binding Domain of Sex-lethal Determined by Multidimensional Heteronuclear Magnetic Resonance" Biochemistry 33, 13775-13786 (1994).
PubMed: 7524663

Assembly members:

Assembly members:
Sex-lethal protein RNA binding domain 2, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pAR-Sxl-RNAP-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sex-lethal protein RNA binding domain 2: MSYARPGGESIKDTNLYVTN LPRTITDDQLDTIFGKYGSI VQKNILRDKLTGRPRGVAFV RYNKREEAQEAISALNNVIP EGGSQPLSVRLAEEHGK

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment

Data type	Count
13C chemical shifts	288
15N chemical shifts	99
1H chemical shifts	608

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Sxl-RBD2	1

Entities:

Entity 1, Sxl-RBD2 97 residues - Formula weight is not available

1

MET

SER

TYR

ALA

ARG

PRO

GLY

GLU

SER

2

ILE

LYS

ASP

THR

ASN

LEU

TYR

VAL

THR

ASN

3

LEU

PRO

ARG

THR

ILE

THR

ASP

GLN

LEU

4

ASP

THR

ILE

PHE

GLY

LYS

TYR

GLY

SER

ILE

5

VAL

GLN

LYS

ASN

ILE

LEU

ARG

ASP

LYS

LEU

6

THR

GLY

ARG

PRO

ARG

GLY

VAL

ALA

PHE

VAL

7

ARG

TYR

ASN

LYS

ARG

GLU

ALA

GLN

GLU

8

ALA

ILE

SER

ALA

LEU

ASN

VAL

ILE

PRO

9

GLU

GLY

SER

GLN

PRO

LEU

SER

VAL

ARG

10

LEU

ALA

GLU

HIS

GLY

LYS

Samples:

sample_one: Sex-lethal protein RNA binding domain 2, [U-15N], 1.5 – 2.0 mM; NaCL 25 mM; d4-acetic_acid 25 mM; NaN3 0.01%; D20 99.996%

sample_two: Sex-lethal protein RNA binding domain 2, [U-15N], 1.5 – 2.0 mM; NaCL 25 mM; d4-acetic_acid 25 mM; NaN3 0.01%; H20 10%; D20 90%

sample_three: Sex-lethal protein RNA binding domain 2, [U-13C; U-15N], 1.5 – 2.0 mM; NaCL 25 mM; d4-acetic_acid 25 mM; NaN3 0.01%; D20 99.996%

sample_four: Sex-lethal protein RNA binding domain 2, [U-13C; U-15N], 1.5 – 2.0 mM; NaCL 25 mM; d4-acetic_acid 25 mM; NaN3 0.01%; H20 10%; D20 90%

sample_conditions: pH: 5.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

No software information available

NMR spectrometers:

Bruker AMX 600 MHz

PDB	1SXL
GB	AAS15801 AAS15802 AAS15803 AAS15804 AAS15805