BMRB Entry 4084

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4084

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Determination of the Secondary Structure and Folding Topology of an RNA Binding Domain of Mammalian hnRNP A1 Protein Using Three-Dimensional Heteronuclear Magnetic Resonance Spectroscopy

Deposition date:

1997-12-23

Original release date:

2001-02-17

Authors:

Garrett, Daniel; Lodi, Patricia; Shamoo, Yousif; Williams, Kenneth; Clore, G.; Gronenborn, Angela

Citation:

Citation: Garrett, Daniel; Lodi, Patricia; Shamoo, Yousif; Williams, Kenneth; Clore, G.; Gronenborn, Angela. "Determination of the Secondary Structure and Folding Topology of an RNA Binding Domain of Mammalian nhRNP A1 Protein Using Three-Dimensional Heteronuclear Magnetic Resonance Spectroscopy" Biochemistry 33, 2852-2858 (1994).

Assembly members:

Assembly members:
human hnRNP A1, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pYS45

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human hnRNP A1: MSKSESPKEPEQLRKLFIGG LSFETTDESLRSHFEQWGTL TDCVVMRDPNTKRSRGFGFV TYATVEEVDAAMNARPHKVD GRVVEPKRAVSRE

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment

Data type	Count
13C chemical shifts	274
15N chemical shifts	92
1H chemical shifts	577

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hnRNP(A1)	1

Entities:

Entity 1, hnRNP(A1) 93 residues - Formula weight is not available

1

MET

SER

LYS

SER

GLU

SER

PRO

LYS

GLU

PRO

2

GLU

GLN

LEU

ARG

LYS

LEU

PHE

ILE

GLY

3

LEU

SER

PHE

GLU

THR

ASP

GLU

SER

LEU

4

ARG

SER

HIS

PHE

GLU

GLN

TRP

GLY

THR

LEU

5

THR

ASP

CYS

VAL

MET

ARG

ASP

PRO

ASN

6

THR

LYS

ARG

SER

ARG

GLY

PHE

GLY

PHE

VAL

7

THR

TYR

ALA

THR

VAL

GLU

VAL

ASP

ALA

8

ALA

MET

ASN

ALA

ARG

PRO

HIS

LYS

VAL

ASP

9

GLY

ARG

VAL

GLU

PRO

LYS

ARG

ALA

VAL

10

SER

ARG

GLU

Samples:

sample_one: human hnRNP A1, [U-15N], 2 mM; D2O 10%; H2O 90%

sample_two: human hnRNP A1, [U-13C; U-15N], 2 mM; D2O 10%; H2O 90%

sample_three: human hnRNP A1, [U-15N], 2 mM; D2O 99.996%

sample_four: human hnRNP A1, [U-13C; U-15N], 2 mM; D2O 99.996%

sample_conditions: pH: 5.7; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

No software information available

NMR spectrometers:

Bruker AM 600 MHz

DBJ	BAG60447 BAG64377
GB	AAP78702 ELK35268
REF	XP_004274506 XP_007659907 XP_012323839