BMRB Entry 4080

Name: NMR Solution Structure of Calcium-Saturated Skeletal Muscle Troponin C
Published: 2001-02-17

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4080

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Solution Structure of Calcium-Saturated Skeletal Muscle Troponin C

Deposition date:

1997-12-07

Original release date:

2001-02-17

Authors:

Slupsky, Carolyn; Sykes, Brian

Citation:

Citation: Slupsky, Carolyn; Sykes, Brian. "NMR Solution Structure of Calcium-Saturated Skeletal Muscle Troponin C" Biochemistry 34, 14953-15964 (1995).

Assembly members:

Assembly members:
Troponin C, polymer, 162 residues, 18000 Da.

Natural source:

Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source:

Experimental source: Production method: E. coli Host organism: Escherichia coli Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Troponin C: ASMTDQQAEARAFLSEEMIA EFKAAFDMFDADGGGDISTK ELGTVMRMLGQNPTKEELDA IIEEVDEDGSGTIDFEEFLV MMVRQMKEDAKGKSEEELAN CFRIFDKNADGFIDIEELGE ILRATGEHVIEEDIEDLMKD SDKNNDGRIDFDEFLKMMEG VQ

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment

Data type	Count
1H chemical shifts	709
13C chemical shifts	272
15N chemical shifts	8

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TnC	1

Entities:

Entity 1, TnC 162 residues - 18000 Da.

1

ALA

SER

MET

THR

ASP

GLN

ALA

GLU

ALA

2

ARG

ALA

PHE

LEU

SER

GLU

MET

ILE

ALA

3

GLU

PHE

LYS

ALA

PHE

ASP

MET

PHE

ASP

4

ALA

ASP

GLY

ASP

ILE

SER

THR

LYS

5

GLU

LEU

GLY

THR

VAL

MET

ARG

MET

LEU

GLY

6

GLN

ASN

PRO

THR

LYS

GLU

LEU

ASP

ALA

7

ILE

GLU

VAL

ASP

GLU

ASP

GLY

SER

8

GLY

THR

ILE

ASP

PHE

GLU

PHE

LEU

VAL

9

MET

VAL

ARG

GLN

MET

LYS

GLU

ASP

ALA

10

LYS

GLY

LYS

SER

GLU

LEU

ALA

ASN

11

CYS

PHE

ARG

ILE

PHE

ASP

LYS

ASN

ALA

ASP

12

GLY

PHE

ILE

ASP

ILE

GLU

LEU

GLY

GLU

13

ILE

LEU

ARG

ALA

THR

GLY

GLU

HIS

VAL

ILE

14

GLU

ASP

ILE

GLU

ASP

LEU

MET

LYS

ASP

15

SER

ASP

LYS

ASN

ASP

GLY

ARG

ILE

ASP

16

PHE

ASP

GLU

PHE

LEU

LYS

MET

GLU

GLY

17

VAL

GLN

Samples:

sample_TnC: Troponin C, [U-15N], 1.4 mM; D2O 10%; H2O 90%; KCl 150 mM; CaCl2 16 mM; TFE 15 % v/v

sample_conditions: pH: 7; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
not available	sample_TnC	not available	sample_conditions

Software:

No software information available

NMR spectrometers:

Varian Unity 600 MHz

PDB	1NCX 1NCY 1NCZ 1TNW 1TNX 1TOP 1YTZ 1YV0 2W49 2W4U 4TNC 5TNC
GB	AAA49097 AAB19538 KFM07723 KFO77465 KFR04283
REF	NP_990781 XP_005240050 XP_005445015 XP_009277803 XP_009277805
SP	P02588 P10246
AlphaFold	P02588 P10246