BMRB Entry 4080

Title:
NMR Solution Structure of Calcium-Saturated Skeletal Muscle Troponin C
Deposition date:
1997-12-07
Original release date:
2001-02-17
Authors:
Slupsky, Carolyn; Sykes, Brian
Citation:

Citation: Slupsky, Carolyn; Sykes, Brian. "NMR Solution Structure of Calcium-Saturated Skeletal Muscle Troponin C"  Biochemistry 34, 14953-15964 (1995).

Assembly members:

Assembly members:
Troponin C, polymer, 162 residues, 18000 Da.

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: E. coli   Host organism: Escherichia coli   Vector: pET3a

Experimental source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: E. coli   Host organism: Escherichia coli   Vector: pET3a

Data sets:
Data typeCount
1H chemical shifts709
13C chemical shifts272
15N chemical shifts8

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TnC1

Entities:

Entity 1, TnC 162 residues - 18000 Da.

1   ALASERMETTHRASPGLNGLNALAGLUALA
2   ARGALAPHELEUSERGLUGLUMETILEALA
3   GLUPHELYSALAALAPHEASPMETPHEASP
4   ALAASPGLYGLYGLYASPILESERTHRLYS
5   GLULEUGLYTHRVALMETARGMETLEUGLY
6   GLNASNPROTHRLYSGLUGLULEUASPALA
7   ILEILEGLUGLUVALASPGLUASPGLYSER
8   GLYTHRILEASPPHEGLUGLUPHELEUVAL
9   METMETVALARGGLNMETLYSGLUASPALA
10   LYSGLYLYSSERGLUGLUGLULEUALAASN
11   CYSPHEARGILEPHEASPLYSASNALAASP
12   GLYPHEILEASPILEGLUGLULEUGLYGLU
13   ILELEUARGALATHRGLYGLUHISVALILE
14   GLUGLUASPILEGLUASPLEUMETLYSASP
15   SERASPLYSASNASNASPGLYARGILEASP
16   PHEASPGLUPHELEULYSMETMETGLUGLY
17   VALGLN

Samples:

sample_TnC: Troponin C, [U-15N], 1.4 mM; D2O 10%; H2O 90%; KCl 150 mM; CaCl2 16 mM; TFE 15 % v/v

sample_conditions: pH: 7; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_TnCnot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • Varian Unity 600 MHz

Related Database Links:

PDB
GB AAA49097 AAB19538 KFM07723 KFO77465 KFR04283
REF NP_990781 XP_005240050 XP_005445015 XP_009277803 XP_009277805
SP P02588 P10246
AlphaFold P10246