BMRB Entry 4074

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4074

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Title:
Backbone 1H, 13C and 15N Chemical Shift Assignments for Reduced Human Ferredoxin
Deposition date:
1997-11-20
Original release date:
2007-03-26
Authors:
Xia, Bin; Volkman, Brian; Markley, John
Citation:

Citation: Xia, Bin; Volkman, Brian; Markley, John. "Evidence for Redidation-State-Dependent Conformational Changes in Human Ferredoxin from Multinuclear, Multidimensional NMR Spectroscopy"  Biochemistry 37, 3965-3973 (1998).

Assembly members:

Assembly members:
human ferredoxin, polymer, 124 residues, 13800 Da.
FE, non-polymer, 55.845 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9609   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: HuFd/pET9a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human ferredoxin: SSSEDKITVHFINRDGETLT TKGKVGDSLLDVVVENNLDI DGFGACEGTLACSTCHLIFE DHIYEKLDAITDEENDMLDL AYGLTDRSRLGCQICLTKSM DNMTVRVPETVADARQSIDV GKTS

Data sets:
Data typeCount
13C chemical shifts285
15N chemical shifts95
1H chemical shifts200

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HuFd-red1
22Fe2S2

Entities:

Entity 1, HuFd-red 124 residues - 13800 Da.

1   SERSERSERGLUASPLYSILETHRVALHIS
2   PHEILEASNARGASPGLYGLUTHRLEUTHR
3   THRLYSGLYLYSVALGLYASPSERLEULEU
4   ASPVALVALVALGLUASNASNLEUASPILE
5   ASPGLYPHEGLYALACYSGLUGLYTHRLEU
6   ALACYSSERTHRCYSHISLEUILEPHEGLU
7   ASPHISILETYRGLULYSLEUASPALAILE
8   THRASPGLUGLUASNASPMETLEUASPLEU
9   ALATYRGLYLEUTHRASPARGSERARGLEU
10   GLYCYSGLNILECYSLEUTHRLYSSERMET
11   ASPASNMETTHRVALARGVALPROGLUTHR
12   VALALAASPALAARGGLNSERILEASPVAL
13   GLYLYSTHRSER

Entity 2, 2Fe2S - Fe - 55.845 Da.

1   FE

Samples:

sample_one: human ferredoxin, [U-99.8% 15N], 1 – 2 mM; Tris-HCl 50 mM

sample_two: human ferredoxin, [U-99% 13C; U-99.8% 15N], 1 – 2 mM; Tris-HCl 50 mM

sample_conditions_HuFd-red: pH: 7.4; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D_1H-15N_HSQCnot availablenot availablesample_conditions_HuFd-red
3D_HNCOnot availablenot availablesample_conditions_HuFd-red
3D_HNCAnot availablenot availablesample_conditions_HuFd-red
3D_HN(CO)CAnot availablenot availablesample_conditions_HuFd-red
3D_HNCACBnot availablenot availablesample_conditions_HuFd-red
3D_CBCA(CO)NHnot availablenot availablesample_conditions_HuFd-red
3D_HCACOnot availablenot availablesample_conditions_HuFd-red
3D_15N-TOCSYdnot availablenot availablesample_conditions_HuFd-red
3D_15N-NOESYdnot availablenot availablesample_conditions_HuFd-red
3D_15N-TOCSYenot availablenot availablesample_conditions_HuFd-red
3D_15N-NOESYnot availablenot availablesample_conditions_HuFd-red

Software:

FELIX v2.30 & 95.0, Molecular Simulations, San Diego, CA - used for all FT of data

Peakpick/PPFLX v1.1, Molecular Simulations, San Diego, CA - used for peakpicking all data

NMR spectrometers:

  • Bruker DMX500 500 MHz
  • Bruker DMX600 600 MHz
  • Bruker DMX750 750 MHz

Related Database Links:

BMRB 4073 4439 4440
PDB
DBJ BAG73145
GB AAA35829 AAA35855 AAA35856 AAA50462 AAA76853
REF NP_004100 XP_001105034 XP_003253520 XP_003910717 XP_003923949
SP P10109
AlphaFold P10109

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks