BMRB Entry 4046

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4046

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Evidence for Similarities between the DNA-Binding Regions of Drosophila melanogaster Heat Shock Factor and the Helix-Turn-Helix and HNF-3/forkhead Families of Transcription Factors

Deposition date:

1997-07-20

Original release date:

1999-08-24

Authors:

Vuister, Geerten; Kim, Soon-Jong; Wu, Carl; Bax, Ad

Citation:

Citation: Vuister, Geerten; Kim, Soon-Jong; Wu, Carl; Bax, Ad. "NMR Evidence for Similarities between the DNA-Binding Regions of Drosophila melanogaster Heat Shock Factor and the Helix-Turn-Helix and HNF-3/forkhead Families of Transcription Factors" (1994).

Assembly members:

Assembly members:
Drosophila Heat Shock Factor, polymer, 136 residues, 15259 Da.

Natural source:

Natural source: Common Name: Fruit Fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHSF33-163

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Drosophila Heat Shock Factor: AAEHSYGDAAAIGSGVPAFL AKLWRIVDDADTNRLICWTK DGQSFVIQNQAQFAKELLPL NYKHNNMASFIRQLNMYGFH KITSIDNGGLRFDRDEIEFS HPFFKRNSPFLLDQIKRKIS NNKNGDDKGVLKPAAE

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment_one

Data type	Count
13C chemical shifts	370
15N chemical shifts	122
1H chemical shifts	454

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	dHSF	1

Entities:

Entity 1, dHSF 136 residues - 15259 Da.

1

ALA

GLU

HIS

SER

TYR

GLY

ASP

ALA

2

ALA

ILE

GLY

SER

GLY

VAL

PRO

ALA

PHE

LEU

3

ALA

LYS

LEU

TRP

ARG

ILE

VAL

ASP

ALA

4

ASP

THR

ASN

ARG

LEU

ILE

CYS

TRP

THR

LYS

5

ASP

GLY

GLN

SER

PHE

VAL

ILE

GLN

ASN

GLN

6

ALA

GLN

PHE

ALA

LYS

GLU

LEU

PRO

LEU

7

ASN

TYR

LYS

HIS

ASN

MET

ALA

SER

PHE

8

ILE

ARG

GLN

LEU

ASN

MET

TYR

GLY

PHE

HIS

9

LYS

ILE

THR

SER

ILE

ASP

ASN

GLY

LEU

10

ARG

PHE

ASP

ARG

ASP

GLU

ILE

GLU

PHE

SER

11

HIS

PRO

PHE

LYS

ARG

ASN

SER

PRO

PHE

12

LEU

ASP

GLN

ILE

LYS

ARG

LYS

ILE

SER

13

ASN

LYS

ASN

GLY

ASP

LYS

GLY

VAL

14

LEU

LYS

PRO

ALA

GLU

Samples:

sample_one: Drosophila Heat Shock Factor, [U-15N], 0.7 – 1.1 mM; D2O 5%; potassium phosphate 10 mM; KCl 50 mM

sample_two: Drosophila Heat Shock Factor, [U-13C; U-15N], 0.7 – 1.1 mM; D2O 5%; potassium phosphate 10 mM; KCl 50 mM

sample_conditions_one: pH: 6.3 na; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

No software information available

NMR spectrometers:

unknown unknown 0 MHz

PDB	1HKS 1HKT
DBJ	BAE16321 BAE16322 BAE16323
GB	AAA28642 AAF57749 AAL13990 ABI31102 ABI31103
REF	NP_001036555 NP_001036556 NP_001036557 NP_476575 XP_002034392
SP	P22813
AlphaFold	P22813