BMRB Entry 4042
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4042
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Citation: Bertini, Ivano; Felli, Isabella; Kastrau, Dieter; Luchinat, Claudio; Piccioli, Mario; Viezzoli, Maria. "Sequence-specific assignment of the 1H and 15N Nuclear Magnetic Resonance
Spectra of the Reduced Recombinant High-potential Iron-sulfur Protein I from
Ectothiorhodospira halophila" Eur. J. Biochem. 225, 703-714 (1994).
Assembly members:
high-potential iron-sulfur protein I, polymer, 73 residues, Formula weight is not available
IRON/SULFUR CLUSTER, non-polymer, 351.640 Da.
Natural source: Common Name: E. halophila Taxonomy ID: 1053 Superkingdom: Eubacteria Kingdom: not available Genus/species: Ectothiorhodospira halophila
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
high-potential iron-sulfur protein I: ASEPRAEDGHAHDYVNEAAD
ASGHPRYQEGQLCENCAFWG
EAVQDGWGRCTHPDFDEVLV
KAEGWCSVYAPAS
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 66 |
| 1H chemical shifts | 425 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | high-potential iron-sulfur protein I | 1 |
| 2 | iron-sulfur_center | 2 |
Entities:
Entity 1, high-potential iron-sulfur protein I 73 residues - Formula weight is not available
| 1 | ALA | SER | GLU | PRO | ARG | ALA | GLU | ASP | GLY | HIS | ||||
| 2 | ALA | HIS | ASP | TYR | VAL | ASN | GLU | ALA | ALA | ASP | ||||
| 3 | ALA | SER | GLY | HIS | PRO | ARG | TYR | GLN | GLU | GLY | ||||
| 4 | GLN | LEU | CYS | GLU | ASN | CYS | ALA | PHE | TRP | GLY | ||||
| 5 | GLU | ALA | VAL | GLN | ASP | GLY | TRP | GLY | ARG | CYS | ||||
| 6 | THR | HIS | PRO | ASP | PHE | ASP | GLU | VAL | LEU | VAL | ||||
| 7 | LYS | ALA | GLU | GLY | TRP | CYS | SER | VAL | TYR | ALA | ||||
| 8 | PRO | ALA | SER |
Entity 2, iron-sulfur_center - Fe4 S4 - 351.640 Da.
| 1 | SF4 |
Samples:
sample_one: high-potential iron-sulfur protein I 6 mM; sodium isoascorbate buffer.15 – 6 mM
sample_conditions_one: pH: .; temperature: 288 K
sample_conditions_two: pH: .; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_one | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker AMX 600 MHz