BMRB Entry 4042

Title:
Sequence-specific Assignment of the 1H and 15N Nuclear Magnetic Resonance Spectra of the Reduced Recombinant High-potential Iron-sulfur Protein I from Ectothiorhodospira halophila
Deposition date:
1997-07-02
Original release date:
1999-10-10
Authors:
Bertini, Ivano; Felli, Isabella; Kastrau, Dieter; Luchinat, Claudio; Piccioli, Mario; Viezzoli, Maria
Citation:

Citation: Bertini, Ivano; Felli, Isabella; Kastrau, Dieter; Luchinat, Claudio; Piccioli, Mario; Viezzoli, Maria. "Sequence-specific assignment of the 1H and 15N Nuclear Magnetic Resonance Spectra of the Reduced Recombinant High-potential Iron-sulfur Protein I from Ectothiorhodospira halophila"  Eur. J. Biochem. 225, 703-714 (1994).

Assembly members:

Assembly members:
high-potential iron-sulfur protein I, polymer, 73 residues, Formula weight is not available
IRON/SULFUR CLUSTER, non-polymer, 351.640 Da.

Natural source:

Natural source:   Common Name: E. halophila   Taxonomy ID: 1053   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Ectothiorhodospira halophila

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
high-potential iron-sulfur protein I: ASEPRAEDGHAHDYVNEAAD ASGHPRYQEGQLCENCAFWG EAVQDGWGRCTHPDFDEVLV KAEGWCSVYAPAS

Data typeCount
15N chemical shifts66
1H chemical shifts425

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1high-potential iron-sulfur protein I1
2iron-sulfur_center2

Entities:

Entity 1, high-potential iron-sulfur protein I 73 residues - Formula weight is not available

1   ALASERGLUPROARGALAGLUASPGLYHIS
2   ALAHISASPTYRVALASNGLUALAALAASP
3   ALASERGLYHISPROARGTYRGLNGLUGLY
4   GLNLEUCYSGLUASNCYSALAPHETRPGLY
5   GLUALAVALGLNASPGLYTRPGLYARGCYS
6   THRHISPROASPPHEASPGLUVALLEUVAL
7   LYSALAGLUGLYTRPCYSSERVALTYRALA
8   PROALASER

Entity 2, iron-sulfur_center - Fe4 S4 - 351.640 Da.

1   SF4

Samples:

sample_one: high-potential iron-sulfur protein I 6 mM; sodium isoascorbate buffer.15 – 6 mM

sample_conditions_one: pH: .; temperature: 288 K

sample_conditions_two: pH: .; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

PDB
SP P04168
AlphaFold P04168