BMRB Entry 4040

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4040

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Main-Chain NMR Assignments for AsiA
Deposition date:
1997-06-30
Original release date:
1999-10-10
Authors:
Urbauer, Jeffrey; Adelman, Karen; Brody, Edward
Citation:

Citation: Urbauer, Jeffrey; Adelman, Karen; Brody, Edward. "Main-Chain NMR Assignments for AsiA"  J. Biomol. NMR 10, 205-206 (1997).

Assembly members:

Assembly members:
Anti-sigma-A, polymer, 90 residues, 10590 Da.

Natural source:

Natural source:   Common Name: bacteriophage t4   Taxonomy ID: 10665   Superkingdom: Viruses   Kingdom: not available   Genus/species: t4-like phages coliphage T4

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pBAS-M1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Anti-sigma-A: MNKNIDTVREIITVASILIK FSREDIVENRANFIAFLNEI GVTHEGRKLNQNSFRKIVSE LTQEDKKTLIDEFNEGFEGV YRYLEMYTNK

Data sets:
Data typeCount
13C chemical shifts251
15N chemical shifts85
1H chemical shifts170

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Anti-sigma-A1

Entities:

Entity 1, Anti-sigma-A 90 residues - 10590 Da.

1   METASNLYSASNILEASPTHRVALARGGLU
2   ILEILETHRVALALASERILELEUILELYS
3   PHESERARGGLUASPILEVALGLUASNARG
4   ALAASNPHEILEALAPHELEUASNGLUILE
5   GLYVALTHRHISGLUGLYARGLYSLEUASN
6   GLNASNSERPHEARGLYSILEVALSERGLU
7   LEUTHRGLNGLUASPLYSLYSTHRLEUILE
8   ASPGLUPHEASNGLUGLYPHEGLUGLYVAL
9   TYRARGTYRLEUGLUMETTYRTHRASNLYS

Samples:

sample_one: Anti-sigma-A, [U-99% 13C; U-99% 15N], 1.5 mM; potassium phosphate 50 mM; sodium azide 0.02%; D2O 10.0%; H2O 90.0%

sample_two: Anti-sigma-A, [U-99% 15N], 2.0 mM; potassium phosphate 50 mM; sodium azide 0.02%; D2O 10.0%; H2O 90.0%

sample_conditions_one: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
DBJ BAI83269
EMBL CUL01214 CUL02094 CUL02264 CUL03022 CUL03542
GB AAA32480 AAD42539 ABI95070 ACP30892 ACP31169
REF NP_049866 YP_002854206 YP_002854584 YP_004415143 YP_006986809
SP P32267
AlphaFold P32267

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks