BMRB Entry 4039
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4039
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Citation: Shao, Xuguang; Sudhof, Thomas; Rizo, Josep. "Assignments of the 1H, 15N and 13C resonances of the calcium-free and
calcium-bound forms of the first C2-domain of synaptotagmin I" J. Biomol. NMR ., .-..
Assembly members:
synaptotagmin I C2A, polymer, 128 residues, 14754 Da.
Natural source: Common Name: rat Taxonomy ID: not available Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Vector: pGex-KG
Entity Sequences (FASTA):
synaptotagmin I C2A: EKLGKLQYSLDYDFQNNQLL
VGIIQAAELPALDMGGTSDP
YVKVFLLPDKKKKFETKVHR
KTLNPVFNEQFTFKVPYSEL
GGKTLVMAVYDFDRFSKHDI
IGEFKVPMNTVDFGHVTEEW
RDLQSAEK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 615 |
| 15N chemical shifts | 134 |
| 1H chemical shifts | 924 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SytI-C2A peptide | 1 |
Entities:
Entity 1, SytI-C2A peptide 128 residues - 14754 Da.
| 1 | GLU | LYS | LEU | GLY | LYS | LEU | GLN | TYR | SER | LEU | ||||
| 2 | ASP | TYR | ASP | PHE | GLN | ASN | ASN | GLN | LEU | LEU | ||||
| 3 | VAL | GLY | ILE | ILE | GLN | ALA | ALA | GLU | LEU | PRO | ||||
| 4 | ALA | LEU | ASP | MET | GLY | GLY | THR | SER | ASP | PRO | ||||
| 5 | TYR | VAL | LYS | VAL | PHE | LEU | LEU | PRO | ASP | LYS | ||||
| 6 | LYS | LYS | LYS | PHE | GLU | THR | LYS | VAL | HIS | ARG | ||||
| 7 | LYS | THR | LEU | ASN | PRO | VAL | PHE | ASN | GLU | GLN | ||||
| 8 | PHE | THR | PHE | LYS | VAL | PRO | TYR | SER | GLU | LEU | ||||
| 9 | GLY | GLY | LYS | THR | LEU | VAL | MET | ALA | VAL | TYR | ||||
| 10 | ASP | PHE | ASP | ARG | PHE | SER | LYS | HIS | ASP | ILE | ||||
| 11 | ILE | GLY | GLU | PHE | LYS | VAL | PRO | MET | ASN | THR | ||||
| 12 | VAL | ASP | PHE | GLY | HIS | VAL | THR | GLU | GLU | TRP | ||||
| 13 | ARG | ASP | LEU | GLN | SER | ALA | GLU | LYS |
Samples:
sample_SytI-C2A_apo: synaptotagmin I C2A, [U-15N; U-13C], 1.0 mM; EGTA 0.2 mM
sample_conditions_set_2: pH: 5.0; temperature: 307 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
| BMRB | 15785 15952 17102 4041 4166 4167 |
| PDB | |
| GB | AAK15077 ELW70522 EPY85163 KFO26677 KFP32561 |
| REF | XP_005976391 XP_010164835 XP_010202276 XP_010211789 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks