BMRB Entry 4038

Title:
Detailed NMR Analysis of the Heme-Protein Interactions in Component IV Glycera Dibranchiata Monomeric Hemoglobin-CO
Deposition date:
1997-06-23
Original release date:
1998-06-13
Authors:
Alam, Steven; Volkman, Brian; Markley, John; Satterlee, James
Citation:

Citation: Alam, Steven; Volkman, Brian; Markley, John; Satterlee, James. "Detailed NMR Analysis of the Heme-Protein Interactions in Component IV Glycera dibranchiata Monomeric Hemoglobin-CO"  J. Biomol. NMR 11, 119-133 (1998).

Assembly members:

Assembly members:
component IV globin of Glycera dibranchiata, polymer, 147 residues, 15021 Da.
PROTOPORPHYRIN IX CONTAINING FE, non-polymer, 616.487 Da.
CARBON MONOXIDE, non-polymer, 28.010 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET9d

Data typeCount
13C chemical shifts581
15N chemical shifts168
1H chemical shifts975

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GMG41
2Heme2
3CO3

Entities:

Entity 1, GMG4 147 residues - 15021 Da.

1   GLYLEUSERALAALAGLNARGGLNVALVAL
2   ALASERTHRTRPLYSASPILEALAGLYSER
3   ASPASNGLYALAGLYVALGLYLYSGLUCYS
4   PHETHRLYSPHELEUSERALAHISHISASP
5   METALAALAVALPHEGLYPHESERGLYALA
6   SERASPPROGLYVALALAASPLEUGLYALA
7   LYSVALLEUALAGLNILEGLYVALALAVAL
8   SERHISLEUGLYASPGLUGLYLYSMETVAL
9   ALAGLUMETLYSALAVALGLYVALARGHIS
10   LYSGLYTYRGLYASNLYSHISILELYSALA
11   GLUTYRPHEGLUPROLEUGLYALASERLEU
12   LEUSERALAMETGLUHISARGILEGLYGLY
13   LYSMETASNALAALAALALYSASPALATRP
14   ALAALAALATYRALAASPILESERGLYALA
15   LEUILESERGLYLEUGLNSER

Entity 2, Heme - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Entity 3, CO - C O - 28.010 Da.

1   CMO

Samples:

sample_one: component IV glycera dibranchiata monomeric hemoglobin-CO, [U-15N;13C]-GMG4, 3.25 mM; H2O 10%; D2O 90%; KCL 100 mM; potasium phosphate buffer 100 mM; CO(g) saturated

sample_conditions_one: pH: 5.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

    Related Database Links:

    PDB
    GB AAA29162 AAB31684
    SP P15447
    AlphaFold P15447

    Download HSQC peak lists in one of the following formats:
    CSV: Backbone or all simulated peaks
    SPARKY: Backbone or all simulated peaks