BMRB Entry 4029

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4029

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Title:
Chemical Shift Mapping of the RNA-binding Interface to the Multiple-RBD Protein Sex-Lethal: Chemical Shifts for the Free Protein
Deposition date:
1997-03-15
Original release date:
1999-10-11
Authors:
Lee, Andrew; Volkman, Brian; Robertson, Stephanie; Rudner, David; Barbash, Daniel; Cline, Thomas; Kanaar, Roland; Rio, Donald; Wemmer, David
Citation:

Citation: Lee, Andrew; Volkman, Brian; Robertson, Stephanie; Rudner, David; Barbash, Daniel; Cline, Thomas; Kanaar, Roland; Rio, Donald; Wemmer, David. "Chemical Shift Mapping of the RNA-binding Interface to the Multiple-RBD Protein Sex-Lethal"  Biochemistry 36, 14306-14317 (1997).

Assembly members:

Assembly members:
Sex-lethal protein, polymer, 184 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruitfly   Taxonomy ID: 7215   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-3b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Sex-lethal protein: MGSDDLMNDPRASNTNLIVN YLPQDMTDRELYALFRAIGP INTCRIMRDYKTGYSFGYAF VDFTSEMDSQRAIKVLNGIT VRNKRLKVSYARPGGESIKD TNLYVTNLPRTITDDQLDTI FGKYGSIVQKNILRDKLTGR PRGVAFVRYNKREEAQEAIS ALNNVIPEGGSQPLSVRLAE EHGK

Data sets:
Data typeCount
13C chemical shifts297
15N chemical shifts149
1H chemical shifts311

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Sex-lethal protein1

Entities:

Entity 1, Sex-lethal protein 184 residues - Formula weight is not available

1   METGLYSERASPASPLEUMETASNASPPRO
2   ARGALASERASNTHRASNLEUILEVALASN
3   TYRLEUPROGLNASPMETTHRASPARGGLU
4   LEUTYRALALEUPHEARGALAILEGLYPRO
5   ILEASNTHRCYSARGILEMETARGASPTYR
6   LYSTHRGLYTYRSERPHEGLYTYRALAPHE
7   VALASPPHETHRSERGLUMETASPSERGLN
8   ARGALAILELYSVALLEUASNGLYILETHR
9   VALARGASNLYSARGLEULYSVALSERTYR
10   ALAARGPROGLYGLYGLUSERILELYSASP
11   THRASNLEUTYRVALTHRASNLEUPROARG
12   THRILETHRASPASPGLNLEUASPTHRILE
13   PHEGLYLYSTYRGLYSERILEVALGLNLYS
14   ASNILELEUARGASPLYSLEUTHRGLYARG
15   PROARGGLYVALALAPHEVALARGTYRASN
16   LYSARGGLUGLUALAGLNGLUALAILESER
17   ALALEUASNASNVALILEPROGLUGLYGLY
18   SERGLNPROLEUSERVALARGLEUALAGLU
19   GLUHISGLYLYS

Samples:

sample_one: Sex-lethal protein, [U-95% 13C; U-95% 15N], 0.5 – 1.0 mM; H2O 90%; D2O 10%; NaCl 30 mM; NaN3 0.02%; sodium phosphate buffer 30 mM

sample_conditions_one: pH: 6.30 na; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions

Software:

xwinnmr v1.1, Bruker - NMR data acquisition

FELIX v2.30, biosym - fourier transformation, peak picking, strip plotting, visualization

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 4028
PDB
GB AAA28884 AAA28921 AAA28922 AAF46240 AAF46241
PIR B39725
REF NP_001027062 NP_001027063 NP_001027065 NP_001162686 NP_001162688
SP P19339
AlphaFold P19339

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks