BMRB Entry 36733
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36733
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Huang, Shu-Yu; Shih, Orion; Jeng, U-Ser; Chang, Chi-Fon; Lin, Jung-Hsin; Malliavin, Therese. "pH Sensitivity of the SERF1a Conformational Ensemble." ACS Omega 11, 2614-2627 (2026).
PubMed: 41585721
Assembly members:
Isoform Short of Small EDRK-rich factor 1, polymer, 64 residues, 7499.576 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: not available Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Isoform Short of Small EDRK-rich factor 1: GSMARGNQRELARQKNMKKT
QEISKGKRKEDSLTASQRKQ
RDSEIMQEKQKAANEKKSMQ
TREK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 169 |
| 15N chemical shifts | 54 |
| 1H chemical shifts | 280 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 64 residues - 7499.576 Da.
| 1 | GLY | SER | MET | ALA | ARG | GLY | ASN | GLN | ARG | GLU | ||||
| 2 | LEU | ALA | ARG | GLN | LYS | ASN | MET | LYS | LYS | THR | ||||
| 3 | GLN | GLU | ILE | SER | LYS | GLY | LYS | ARG | LYS | GLU | ||||
| 4 | ASP | SER | LEU | THR | ALA | SER | GLN | ARG | LYS | GLN | ||||
| 5 | ARG | ASP | SER | GLU | ILE | MET | GLN | GLU | LYS | GLN | ||||
| 6 | LYS | ALA | ALA | ASN | GLU | LYS | LYS | SER | MET | GLN | ||||
| 7 | THR | ARG | GLU | LYS |
Samples:
sample_1: serf1a, [U-13C; U-15N], 0.5 mM; NaN3 0.02%; NaCl 20 mM; NaPi 20 mM; H2O 90%; D2O, [U-2H], 10%
sample_conditions_1: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation
Sparky, Goddard - peak picking
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks