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PDB ID: 9m27
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36732
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Name: SERF1_HUMAN short isoform of Small EDRK-rich factor 1, SERF1a at pH 6.0.
Published: 2026-03-26

Title:

SERF1_HUMAN short isoform of Small EDRK-rich factor 1, SERF1a at pH 6.0.

Deposition date:

2025-02-27

Original release date:

2026-03-26

Authors:

Huang, S.; Shih, O.; Jeng, U.; Chang, C.; Lin, J.; Malliavin, T.

Citation:

Citation: Huang, Shu-Yu; Shih, Orion; Jeng, U-Ser; Chang, Chi-Fon; Lin, Jung-Hsin; Malliavin, Therese. "pH Sensitivity of the SERF1a Conformational Ensemble." ACS Omega 11, 2614-2627 (2026).
PubMed: 41585721

Assembly members:

Assembly members:
Isoform Short of Small EDRK-rich factor 1, polymer, 64 residues, 7499.576 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: not available Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Isoform Short of Small EDRK-rich factor 1: GSMARGNQRELARQKNMKKT QEISKGKRKEDSLTASQRKQ RDSEIMQEKQKAANEKKSMQ TREK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	185
15N chemical shifts	72
1H chemical shifts	444

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 64 residues - 7499.576 Da.

1

GLY

SER

MET

ALA

ARG

GLY

ASN

GLN

ARG

GLU

2

LEU

ALA

ARG

GLN

LYS

ASN

MET

LYS

THR

3

GLN

GLU

ILE

SER

LYS

GLY

LYS

ARG

LYS

GLU

4

ASP

SER

LEU

THR

ALA

SER

GLN

ARG

LYS

GLN

5

ARG

ASP

SER

GLU

ILE

MET

GLN

GLU

LYS

GLN

6

LYS

ALA

ASN

GLU

LYS

SER

MET

GLN

7

THR

ARG

GLU

LYS

Samples:

sample_1: SERF1_HUMAN short isoform of Small EDRK-rich factor 1, SERF1a, [U-13C; U-15N], 0.5 mM; NaN3 0.02%; NaCl 20 mM; NaPi 20 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 20 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

Sparky, Goddard - chemical shift assignment

Sparky, Goddard - peak picking

NMR spectrometers:

Bruker Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 36732