BMRB Entry 36724
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36724
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Biswas, Karishma; Mitra, Sudipta; Roy, Dibakar; Roy, Sanhita; Sarkar, Dibakar; Son, DeokHyun; Das, Rohit; Roy, Anuradha; Senapati, Dulal; Ilyas, Humaira; Harikishore, A; Biswas, Ranjit; Chakrabarty, Suman; Lee, DongKuk; Biswas, Indranil; Saha, Sudipto; Kundu, Pallob; Bhunia, Anirban. "Transgenic Tobacco Plants Expressing Synthetic Peptides: A Functional and Structural Analysis for Pathogen Resistance." Plant Biotechnol. J. 24, 526-546 (2026).
PubMed: 40952904
Assembly members:
VR18 (VAL-ALA-ARG-GLY-TRP-GLY-ARG-LYS-CYS-PRO-LEU-PHE-GLY-LYS-ASN-LYS-SER-ARG), polymer, 18 residues, 2066.479 Da.
Natural source: Common Name: Dengue virus Taxonomy ID: 12637 Superkingdom: Viruses Kingdom: not available Genus/species: Orthoflavivirus denguei
Experimental source: Production method: chemical synthesis Host organism: unidentified
Entity Sequences (FASTA):
VR18 (VAL-ALA-ARG-GLY-TRP-GLY-ARG-LYS-CYS-PRO-LEU-PHE-GLY-LYS-ASN-LYS-SER-ARG): VARGWGRKCPLFGKNKSR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 88 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 18 residues - 2066.479 Da.
| 1 | VAL | ALA | ARG | GLY | TRP | GLY | ARG | LYS | CYS | PRO | ||||
| 2 | LEU | PHE | GLY | LYS | ASN | LYS | SER | ARG |
Samples:
sample_1: VR18 (VAL-ALA-ARG-GLY-TRP-GLY-ARG-LYS-CYS-PRO-LEU-PHE-GLY-LYS-ASN-LYS-SER-ARG) 0.5 mM; LIPOPOLYSACCHARIDE BICELLE 0.06 mM; H2O 90%; D2O, [U-2H], 10%
sample_conditions_1: ionic strength: 0 mM; pH: 4; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TopSpin v4.0.6, Bruker Biospin - collection
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure calculation
Sparky v3.114, Goddard - chemical shift assignment
TopSpin v4.0.6, Bruker Biospin - processing
CYANA v3.114, Guntert, Mumenthaler and Wuthrich - refinement
NMR spectrometers:
- Bruker AVANCE III HD 700 MHz