BMRB Entry 36569

Name: Another hairpin structure found in the RNA element involved in piRNA biogenesis
Published: 2025-11-04

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PDB ID: 8jhp
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36569
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Another hairpin structure found in the RNA element involved in piRNA biogenesis

Deposition date:

2023-05-25

Original release date:

2025-11-04

Authors:

Takase, N.; Kawai, G.

Citation:

Citation: Takase, N.; Kawai, G.. "Another hairpin structure found in the RNA element involved in piRNA biogenesis" .

Assembly members:

Assembly members:
RNA (27-MER), polymer, 27 residues, 8642.199 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: 7227 Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: chemical synthesis Host organism: unidentified

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA (27-MER): ACUCGAAAGAACAUGUUUUC CAAGAGU

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
1H chemical shifts	107

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1

Entities:

Entity 1, entity_1 27 residues - 8642.199 Da.

1

A

C

U

C

G

A

G

A

2

A

C

A

U

G

U

C

3

C

A

G

A

G

U

Samples:

sample_1: RNA (27-MER) 0.13 mM; NaCl 50 mM; Na2HPO4/NaH2PO4 20 mM; H2O 95%; D2O, [U-2H], 5%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H HOHAHA	sample_1	isotropic	sample_conditions_1

Software:

Sparky v3.114, Goddard - chemical shift assignment

CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation

Sparky v3.114, Goddard - peak picking

NMR spectrometers:

Bruker AVANCE NEO 600 MHz